Mouse Bone Marrow Derived Macrophage (mBMDM)

Description

Product Description

Macrophages are a type of white blood cell differentiated from circulating bone marrow-derived monocytes. Macrophages are responsible for detecting, engulfing and digesting cellular debris, apoptotic cells, and invading pathogens in a process called phagocytosis. Macrophages can be identified by several specific cell surface proteins including CD11b, CD14, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by immunohistochemistry or flow cytometry analysis ^[1]. T Bone marrow-derived macrophages are suitable for numerous applications including phagocytosis, gene expression profiling, and Morphological examination of cytospins using histological stains (e.g., May-Grünwald-Giemsa staining), etc.^[2].

iXCells Biotechnologies provides Mouse Bone Marrow Derived Macrophage (mBMDM), which were differentiated in the presence of M-CSF using the bone marrow cells isolated from adult C57BL/6 mice (Figure 1). mBMDM are harvested at P0 and delivered freshly or frozen. For frozen cells, each vial contains ≥ 5 million cells in 1 mL volume. mBMDMs are characterized by flow cytometry analysis with >97% CD11b+ purity (Figure 2). mBMDMs are negative for mycoplasma, bacteria, yeast, and fungi. It is recommended to use Macrophage Culture Medium (Cat # MD-0097) for in vitro culturing of mBMDM. However, mBMDMs are not recommended for long-term cultures or further expansion.

Figure 1. Mouse Bone Marrow Derived Macrophage (mBMDM). (A) Phase contrast images. (B) The purity of the mBMDM was measured using CD11b+ antibody by flow cytometry analysis.

Product Details

References

[1]. Cline MJ and Sumner MA. Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series. Blood. 1972; 40: 62-69.

[2]. Wynn TA and Barron L. Macrophages: master regulators of inflammation and fibrosis. Semin Liver Dis. 2010; 30: 245-57.

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