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Human Adipose Derived Stem Cells (hADSC, Normal)

Human Adipose Derived Stem Cells (hADSC, Normal)

Catalog Number: 10HU-001
Availability: In Stock
Price: $399.00

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Product Description

Normal Human Adipose-Derived Stem cells (ADSCs) are isolated from normal human lipoaspirate tissue collected during elective surgical liposuction procedures or dermal adipose tissues. It has been shown that the ADSCs demonstrate very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells. Thousands of articles have been published on ADSCs using a variety of terminology, including preadipocytes, adipose-derived mesenchymal stem cells (AD-MSCs), adipose MSCs (AMSCs), adipose-derived adult stem (ADAS) cells, and adipose stromal/stem cells (ASCs). Normal human ADSCs have been reported to differentiate into many different lineage including chondrogenic, osteogenic, adipogenic and neural. And have been applied in studies include stem cell differentiation, regenerative medicine [1], and cell therapy [2].

Each lot of iXCells normal human ADSCs originates from a single donor of human lipoaspirate tissue, and then cryopreserved at passage one. hADSCs are positive for CD29, CD44, CD73, CD90, CD105, and negative for CD14, CD31, and CD45 (Figure 1). hADSC can be in vitro differentiated into adipocytes and osteoblasts (Figure 2 and 3) using Adipocyte Differentiation Medium (Cat # MD-0005) and Osteogenic Differentiation Medium (Cat # MD-0006). hADSC can be further expanded for 3-4 passages using Adipose-Derived Stem Cell Growth Medium (Cat# MD0003)Pertinent additional donor information, including Body Mass Index (BMI) may be available upon request. All the cells provided by iXCells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C.  A Certificate of Analysis is provided for each cell lot purchased.

Diseased state ADSCs from donors with Type 1 Diabetes (T1D), Type 2 Diabetes (T2D), Alzheimers's Disease (AD), Parkinson's Disease (PD)Amyotrophic Lateral Sclerosis (ALS) are available as separate products. 

Human ADSCs

Figure 1.  Immunostaining of cell surface markers of human ADSCs.

Adipogenic Induction

Figure 2. Human ADSC adipocyte differentiation (Day 14 post adipogenic induction).

Osteogenic induction

Figure 3. Human ADSC osteogenic induction (Day 24 post osteogenic induction). Alizarin Red S staining of osteoblasts. The extracellular calcium deposit was stained in bright orange-red color.

Product Details

  Tissue

  Subcutaneous white fat, or dermal adipose tissues from normal donors

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Adipose-Derived Stem Cell Growth Medium (Cat# MD0003) 

  Adipocyte Differentiation Medium (Cat# MD-0005)

  Osteogenic Differentiation Medium (Cat# MD-0006)

References

[1]  Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.

[2]  Bertheuil N, et al and Tarte K. Adipose-derived stromal cells: history, isolation, immunomodulatory properties and clinical perspective. Ann Chir Plast Esthet. 2015;60(2): 94-102.

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[1]  Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.

[2]  Bertheuil N, et al and Tarte K. Adipose-derived stromal cells: history, isolation, immunomodulatory properties and clinical perspective. Ann Chir Plast Esthet. 2015;60(2): 94-102.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

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