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Human Brain Microvascular Endothelial Cells (HBMVEC)

Human Brain Microvascular Endothelial Cells (HBMVEC)

Catalog Number: 10HU-051
Availability: In Stock
Price: $549.00

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Product Description

The brain microvascular endothelial cells (HBMVEC) are the major element of the blood-brain barrier and comprise the primary limitation to passage of substances, both soluble and cellular, from the blood into the brain. HBMVEC utilizes unique features that distinguish themselves from those of peripheral endothelial cells. Most prominent among these are the following: 1) intercellular ‘tight junctions’ that display high transendothelial electrical resistance and retard paracellular flux [1], 2) absence of fenestrae and a reduced level of fluid-phase endocytosis [2], and 3) asymmetrically-localized enzymes and carrier-mediated transport systems that engender a truly ‘polarized’ phenotype [3]. Like peripheral endothelial cells, however, HBMVEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. HBMVEC has been widely used for studying the molecular and cellular basis of blood-brain barrier.

HBMVEC from iXCells Biotechnologies are isolated from human brain tissue. HBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBMVEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HBMVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBMVEC are guaranteed to further expand for 10 population doublings in Endothelial Cell Growth Medium (Cat# MD-0010).

Human Brain Microvascular Endothelial Cells (HBMVEC)

Figure 1. Immunostaining of Human Brain Microvascular Endothelial Cells (HBMVEC) using the antibodies against vWF (green) and CD31 (red).

Product Details

  Tissue

  Human brain tissue

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

References

[1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55. 
[2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14. [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96.

[1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55. 
[2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14. [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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