Welcome visitor you can login or create an account.

Shopping Cart

0 item(s) - $0.00
Your shopping cart is empty!
Human Colonic Microvascular Endothelial Cells (HCoMEC)

Human Colonic Microvascular Endothelial Cells (HCoMEC)

Catalog Number: 10HU-055
Availability: In Stock
Price: $599.00

Available Options

* Package Size (Required):

Qty:     - OR -   Add to Wish List
Add to Compare

Product Description

Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation [1]. Pharmacological inhibition of NOS in activated intestinal microvascular endothelial cells resulted in a significant increase in leukocytes binding [2]. Gene expression profile studies revealed that intestinal endothelial cells express biotinidase, which is involved in biotin recycling [3]. Human colonic microvascular endothelial cell (HCoMEC) cultures have enabled scientists to perform systematic analyses of cytokine profiles with regard to mRNA expression and protein secretion, and to compare these data with cytokine profiles from other endothelial cells.

HCoMEC from iXCells Biotechnologies are isolated from human colonic tissue. HCoMEC are cryopreserved at passage one and delivered frozen. Each vial contains >0.5 million cells in 1 ml volume. HCoMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM) and by uptake of DiI-Ac-LDL. HCoMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoMEC are guaranteed to further expand for 10 population doublings in Endothelial Cell Growth Medium (Cat# MD-0010)

Product Details

  Tissue

  Human colonic tissue

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

References

[1]. Ogawa, H., Rafiee, P., Heidemann, J., Fisher, P. J., Johnson, N. A., Otterson, M. F., Kalyanaraman, B., Pritchard, K. A. Jr., Binion, D. G. (2003) Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cells. J Immunol. 170(12):5956-64. 
[2]. Binion, D. G., Fu, S., Ramanujam, K. S., Chai, Y. C., Dweik, R. A., Drazba, J. A., Wade, J. G., Ziats, N. P., Erzurum, S. C. and Wilson, K. T. (1998) iNOS xpression in human intestinal microvascular endothelial cells inhibits leukocyte adhesion. Am. J. Physiol. 275:G592-603.
[3]. Chi, J-T, Chang, H. Y., Haraldsen, G., Jahnsen, F. L., Troyanskaya, O. G., Chang, D. S., Wang, Z., Rockson, S. G., van de Rijn, M., Botstein, D. and Brown, P. O. (2003) Endothelial cell diversity revealed by global expression profiling. PNAS 100(19):10623-10628.

[1]. Ogawa, H., Rafiee, P., Heidemann, J., Fisher, P. J., Johnson, N. A., Otterson, M. F., Kalyanaraman, B., Pritchard, K. A. Jr., Binion, D. G. (2003) Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cells. J Immunol. 170(12):5956-64. 
[2]. Binion, D. G., Fu, S., Ramanujam, K. S., Chai, Y. C., Dweik, R. A., Drazba, J. A., Wade, J. G., Ziats, N. P., Erzurum, S. C. and Wilson, K. T. (1998) iNOS xpression in human intestinal microvascular endothelial cells inhibits leukocyte adhesion. Am. J. Physiol. 275:G592-603.
[3]. Chi, J-T, Chang, H. Y., Haraldsen, G., Jahnsen, F. L., Troyanskaya, O. G., Chang, D. S., Wang, Z., Rockson, S. G., van de Rijn, M., Botstein, D. and Brown, P. O. (2003) Endothelial cell diversity revealed by global expression profiling. PNAS 100(19):10623-10628.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

Related Products (1)
Name Description Catalog number
Endothelial Cell Growth Medium MD-0010