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Human Conjunctival Epithelial Cells (HConEpC)

Human Conjunctival Epithelial Cells (HConEpC)

Catalog Number: 10HU-097
Availability: Out Of Stock
Price: $519.00

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Product Description

The human conjunctiva is a translucent membrane that lines the inner surface of the eyelids and covers the sclera. It is overlaid by two layers of stratified, non-keratinized conjunctival epithelial cells (HConEpC). HConEpC can be distinguished from the corneal epithelium by a specific expression profile of keratins [1]. HConEpC is covered by mucin-rich glycocalyx, which promotes tear adherence, prevents pathogen penetrance, and provides lubrication. Decrease or loss of mucin/glycocalyx production generates squamous metaplasia, which may lead to dry eye and ocular surface diseases [2]. HConEpC also respond to histamine by stimulating phosphatidylinositol turnover and secreting cytokines, and as a result may be a good target when developing topical ocular drugs to treat allergic conjunctivitis [3].

iXCells Biotechnologies provides high quality HConEpC, which are isolated from human conjunctiva and cryopreserved at P1, with >0.5 million cells in each vial. HConEpC express cytokeratin-18 and -19 and are characterized by their cobble stone morphology in serum-free culture. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 10 population doublings in Corneal Epithelial Cell Growth Medium (Cat# MD-0064) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human conjunctiva

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Corneal Epithelial Cell Growth Medium (Cat# MD-0064)

References

[1]. Meller D., Pires R. T. and Tseng S. C. (2002) Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br.J.Ophthalmol. 86:463-471. 
[2]. Paladino G., Marino C., La Terra Mule s., Civiale C., Rusciano D. and Enea V (2004) Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva. Tissue Cell 36:323-332. 
[3]. John M. Yanni, Lori K. Weimer, Najam A. Sharif, Shou X. Xu, Daniel A. Gamache, Joan M. Spellman, (1999) Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular. Allergy Drugs. Arch Ophthalmol. 117:643-647.

[1]. Meller D., Pires R. T. and Tseng S. C. (2002) Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br.J.Ophthalmol. 86:463-471. 
[2]. Paladino G., Marino C., La Terra Mule s., Civiale C., Rusciano D. and Enea V (2004) Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva. Tissue Cell 36:323-332. 
[3]. John M. Yanni, Lori K. Weimer, Najam A. Sharif, Shou X. Xu, Daniel A. Gamache, Joan M. Spellman, (1999) Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular. Allergy Drugs. Arch Ophthalmol. 117:643-647.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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