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Human Hepatic Stellate Cells- Adult (HHSC)

Human Hepatic Stellate Cells- Adult (HHSC)

Catalog Number: 10HU-210
Availability: In Stock
Price: $789.00

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Product Description

Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCs play vital roles in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinol metabolism, storage and release. The stellate cell is the major cell type involved in liver fibrosis in response to liver injury. In healthy liver, HSCs are in a quiescent state, and contains numerous vitamin A lipid droplets, constituting the largest reservoir of vitamin A in the body. When the liver is damaged, HSCs can change into an activated state, which is characterized by proliferation, contractility and chemotaxis. The amount of vitamin A decreases progressively in injured liver. The activated HSCs also secrete collagen scar tissue, which can lead to cirrhosis. In chronic liver disease, prolonged and repeated activation of stellate cells causes liver fibrosis [1,2]. Primary culture of HSC is a valuable tool to study liver fibrosis. 

iXCells Biotechnologies provides high quality Human Hepatic Stellate Cells (HHSC), which are isolated from adult human liver and cryopreserved with >1 million cells in each vial. Each vial is sufficient to seed on four 10 cm dishes, and cells will be ready in 5~6 days to be passaged at 1:3 ratio. HUSMC express Desmin and GFAP, and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung. HUSMC can further expand for 2-3 population doublings in Stellate Cell Growth Medium (Cat # MD-0014) under the condition suggested by iXCells Biotechnologies.

Human Hepatic Stellate Cells

Figure 1. Human hepatic stellate cells (HHSC) were stained with HSC markers, including Desmin and GFPA as indicated (green).  Phase contrast images of each staining is shown on the right panel.  

Product Details

  Tissue

  Adult human liver

  Package Size

  1.0 million cells/vial  

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Stellate Cell Growth Medium (Cat # MD-0014)

References

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.
[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.
[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Stellate Cell Growth Medium MD-0014