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Human Peripheral Blood CD14+ Monocytes

Human Peripheral Blood CD14+ Monocytes

Catalog Number: 10HU-008
Availability: In Stock
Price: $599.00

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Product Description

Monocytes are a type of white blood cells (leukocytes). Monocytes constitute 10~30% of peripheral blood mononuclear cells in the human body. They play multiple roles in immune function including replenishing resident macrophages under normal states. And in response to inflammation signals, monocytes can move quickly (approx. 8–12 hours) to sites of infection in the tissues and divide/differentiate into macrophages and dendritic cells to elicit an immune response [1]. CD14 is an important surface marker for monocytes.

iXCells Biotechnologies offers CD14+ monocytes isolated from normal human peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic selection.  > 92% of the cells are CD14+ as showed by flow cytometric analysis (Figure 1). 

CD14+ monocytes

Figure 1. Flow cytometric analysis showed A. >96% of purified cells are CD14 positive. B. C. Flow cytometric analysis of monocytes dendritic cell differentiation, showed >53% of cell are CD83 positive and >77% cell are CD1a positive.

Product Details

  Tissue

  Normal human peripheral blood

  Package Size

  1.0x107 cells/vial, 4.0x107 cells/vial,

  Purity

  >92%

  Passage Number

  P0

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Suspension

  Media

  Blood Cell Culture Medium (Cat# MD-0007)

 

References

[1]  Ziegler-Heitbrock, L (2007). "The CD14+ CD16+ Blood Monocytes: their Role in Infection and Inflammation, Review". J Leukocyte Biology 81 (3): 584–92.

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[1]  Ziegler-Heitbrock, L (2007). "The CD14+ CD16+ Blood Monocytes: their Role in Infection and Inflammation, Review". J Leukocyte Biology 81 (3): 584–92.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Blood Cell Culture Medium MD-0007
Monocytes DC Differentiation Medium MD-0008
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