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Human Aortic Adventitial Fibroblasts (HAAF)

Human Aortic Adventitial Fibroblasts (HAAF)

Catalog Number: 10HU-120
Availability: In Stock
Price: $599.00

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Product Description


In vascular adventitia, the outermost connective tissue covering the vessel, adventitial fibroblasts (AF) produce collagen to provide structural support by anchoring the blood vessel to nearby tissues. AF are the first cells of the vascular wall to respond to hypertension and vascular injury through activation and proliferation [1]. During pathological conditions, AF produce cytokines and chemokines to induce mass infiltration of immune cells into the adventitial layer of the vessel wall. Immune cell infiltration into the adventitia results in adventitial inflammation and can lead to cardiovascular disease [2, 3]. The important properties of AF make AF cultures an ideal tool for studying the pathogenesis of cardiovascular disease and for the development of novel disease treatments.They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.

Human Aortic Adventitial Fibroblasts (HAAF) from iXCells Biotechnologies are isolated from human aortic artery. HAAF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAAF are characterized by spindle morphology and by immunofluorescence with antibody specific to fibronectin. HAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAAF are guaranteed to further expand for 12 population doublings in Fibroblast Growth Medium (Cat# MD-0011)

Product Details

  Tissue

  Human aortic artery

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Fibroblast Growth Medium (Cat# MD-0011)

References

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. 
[2] Pendurthi, U. R., Rao, L. V. M., Williams, J. T. and Idell, S. (1999) Regulation of tissue factor pathway inhibitor expression in smooth muscle cells. Blood 94:579-586. 
[3] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86.

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. 
[2] Pendurthi, U. R., Rao, L. V. M., Williams, J. T. and Idell, S. (1999) Regulation of tissue factor pathway inhibitor expression in smooth muscle cells. Blood 94:579-586. 
[3] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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