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Human Epidermal Melanocytes-adult (HEM-a)

Human Epidermal Melanocytes-adult (HEM-a)

Catalog Number: 10HU-167
Availability: Out Of Stock
Price: $399.00

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Product Description

Melanocytes are neural crest-derived cells that produce melanin via melanogenesis. Melanocytes localize to several tissues including the epidermis, eye, inner ear and leptomeninges. Dysregulation of melanocyte migration, proliferation, or survival during embryonic development thus causes congenital disorders in those tissues as seen in Tietz syndrome, Waardenburg syndrome, and piebaldism [1]. In the bottom layer of skin epidermis, melanocytes synthesize and transfer dark-colored melanin to surrounding keratinocytes to give skin pigmentation. Melanin also blocks UV-B light to protect the hypodermis from solar exposure-induced photodamage. Progress in culture techniques, along with an improved understanding of melanocyte biology, has led to a successful culture system to model melanomas, inner ear homeostasis, vitiligo, and mitochondrial dysfunction in Duchenne Muscular Dystrophy [2-4].

iXCells Biotechnologies provides high quality Human Epidermal Melanocytes-adult (HEM-a), which are isolated from adult human skin and cryopreserved at P1, with >0.5 million cells in each vial. HEM-a express fibronectin and NGF-receptor (p75). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 12 population doublings in Melanocyte Growth Medium (Cat# MD-0049) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Adult human skin

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Melanocyte Growth Medium (Cat# MD-0049)

References

[1]. Eisinger, M. and Marko, O. (1982) Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proc. Natl. Acad. USA 79:2018-2022. 
[2]. Tang, A., Eller, M. S., Hara, M., Yaar, M., Hirohashi, S. and Gilchrest, B. A. (1994) E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. J. Cell Sci. 107:983-992. 
[3]. Shioda, T., Fenner, M. H. and Isselbacher, K. J. (1996) msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation. Proc. Natl. Acad. Sci. USA 93:12298-12303.

[1]. Eisinger, M. and Marko, O. (1982) Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin. Proc. Natl. Acad. USA 79:2018-2022. 
[2]. Tang, A., Eller, M. S., Hara, M., Yaar, M., Hirohashi, S. and Gilchrest, B. A. (1994) E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. J. Cell Sci. 107:983-992. 
[3]. Shioda, T., Fenner, M. H. and Isselbacher, K. J. (1996) msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation. Proc. Natl. Acad. Sci. USA 93:12298-12303.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Melanocyte Growth Medium MD-0049