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Human Mammary Fibroblasts (HMF)

Human Mammary Fibroblasts (HMF)

Catalog Number: 10HU-138
Availability: Out Of Stock
Price: $579.00

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Product Description

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Primary human mammary fibroblasts (HMF) synthesize components of the stromal extracellular matrix of the mammary gland. More importantly, such stromal extracellular matrix regulates the proliferation and differentiation of mammary epithelial cells by influencing their gene expression [1]. In addition, previous studies have shown that mammary fibroblasts play a role in recruiting macrophages for immune surveillance which, in concert with extracellular matrix turnover and angiogenesis, is associated with a supportive mammary microenvironment for tumor cell progression [2, 3]. These results suggest that HMF play a stimulatory functional role in breast cancer development and invasion.

iXCells Biotechnologies provides high quality HMF, which are isolated from human breast and cryopreserved at P0, with >0.5 million cells in each vial. HMF express fibronectin, CD90, and vimentin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 16 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human breast

  Package Size

  0.5 million cells/vial  

  Passage Number

  P0

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Fibroblast Growth Medium (Cat# MD-0011)

References

[1] D Zangani, KM Darcy, PA Masso-Welch, ES Bellamy, MS Desole, MM Ip (1999) Multiple differentiation pathways of rat mammary stromal cells in vitro: acquisition of a fibroblast, adipocyte or endothelial phenotype is dependent on hormonal and extracellular matrix stimulation. Differentiation. 64(2)91-101. 
[2] WG Jiang, D Grimshaw, TA Martin, G Davies, C Parr, G Watkins, J Lane, R Abounader, J Laterra and RE Mansel (2003) Reduction of Stromal Fibroblast-induced Mammary Tumor Growth, by Retroviral Ribozyme Transgenes to Hepatocyte Growth Factor/Scatter Factor and its Receptor, c-MET. Clinical Cancer Research (9)4274-4281.

[1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9. 
[2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54. 
[3] Burgess, M. L., Terracio, L, Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Fibroblast Growth Medium MD-0011