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Starting at: $719.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| 10HU-112-0.5M | Cryopreserved, 0.5 million cells/vial | Starting at: $719.00 |
Product Description
Human renal proximal tubular epithelial cells (HRPTEpC) play a central role in renal physiology. They reabsorb the substances including the glucose and amino acids as well as control acid-base balance by the excretion of almost all the bicarbonate and the synthesis of ammonia. They are also involved in the excretion of metabolic end products. Furthermore, these cells are particularly sensitive to ischemic injury, and represent a primary target for xenobiotics, such as nephrotoxins (and their metabolites), whose effects can extend up to the kidney failure [1, 2]. HRPTEpC express IL-2R alpha and MHC class II antigens during inflammation, after renal transplantation, or in crescentic glomerulonephritis [3]. HRPTEpC culture provides a valuable in vitro model for studying the mechanisms of proximal tubular cell physiology and pathophysiology, as well as the potential mechanisms underlying nephrotoxins-induced renal toxicity.
iXCells Biotechnologies provides high quality HRPTEpC, which are isolated from human kidneys and cryopreserved at P1, with ≥ 0.5 million cells in each vial. HRPTEpC express cytokeratin-18, -19, vimentin and ZO-1[4]. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRPTEpC can be expanded for no more than 3 passages using Epithelial Cell Growth Medium (Cat# MD-0041) under the conditions provided by iXCells Biotechnoliges. Further expansion may decrease the purity of the epithelial population.
Figure 1. Human renal proximal tubular epithelial cells (HRPTEpC). (A) Phase contrast image of HPRTEpC. (B & C) Immunofluorescence staining with antibodies against ZO-1 (B) and Megalin (C).
Product Details
| Tissue | Normal Human kidney |
| Package Size | 0.5 million cells/vial |
| Passage Number | P1 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Epithelial Cell Growth Medium (Cat# MD-0041) |
References
[1] van Kooten, C., van der Linde, X., Woltman, A. M., van Es, L. A. and Daha, M. R. (1999) “Synergistic effect of interleukin-1 and CD40L on the activation of human renal tubular epithelial cells”. Kidney Int 56(1):41-51.
[2] Schmouder, R. L., Strieter, R. M., Wiggins, R. C., Chensue, S. W. and Kunkel, S. L. (1992) “In vitro and in vivo interleukin-8 production in human renal cortical epithelia”. Kidney Int 41(1):191-8.
[3] Wuthrich, R. P., Glimcher, L. H., Yui, M. A., Jevnikar, A. M., Dumas, S. E. and Kelley, V. E. (1990) “MHC class II, antigen presentation and tumor necrosis factor in renal tubular epithelial cells”. Kidney Int 37(2):783-92.
[4] Cynthia Van der Hauwaert ,Grégoire Savary ,Viviane Gnemmi ,François Glowacki ,Nicolas Pottier,Audrey Bouillez,Patrice Maboudou,Laurent Zini,Xavier Leroy,Christelle Cauffiez,Michaël Perrais ,Sébastien Aubert. (2013) “Isolation and Characterization of a Primary Proximal Tubular Epithelial Cell Model from Human Kidney by CD10/CD13 Double Labeling”. PLOS One 8(6): e66750
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