Human Prostate Epithelial Cells (HPrEpC)

Product Description

The prostate is a major accessory organ of the male mammalian reproductive system, with structural and functional activities under the control of androgens. The prostate is composed of three compartments, which can be identified by the cytokeratin expression profile [1]. The basal cells express predominantly high molecular weight cytokeratins (CK5 and CK14), the luminal secretory cells express predominantly low molecular weight cytokeratins (CK8 and CK18) and the intermediate cells express a combination of basal and luminal cytokeratins [2]. Additionally, there is increasing evidence that basal epithelial cells differentiate into luminal epithelial cells. Prostate cancer is one of the most common cancers affecting men and it has a high morbidity and mortality rate. Human primary prostate epithelial cell (HPrEpiC) culture provides unique opportunities to study many important features of the prostate, as well as, chemical and hormonal carcinogenesis.

HPrEpC from iXCells Biotechnologies are isolated from normal human prostate tissue. HPrEpC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPrEpC are characterized by immunofluorescence with antibodies specific to cytokeratine (CK-14, -18 and -19). HPrEpC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPrEpC are guaranteed to further expand for 12 population doublings in Epithelial Cell Growth Medium (Cat# MD-0041).

Product Details

References

[1] van Leenders G, Dijkman H, Hulsbergen-van de Kaa C, Ruiter D, Schalken J: Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab Invest 2000, 80:1251-1258.
[2] Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C: Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 1990, 143:167-171.

[1] van Leenders G, Dijkman H, Hulsbergen-van de Kaa C, Ruiter D, Schalken J: Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab Invest 2000, 80:1251-1258.
[2] Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C: Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 1990, 143:167-171.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.