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Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Catalog Number: 10HU-076
Availability: Out Of Stock
Price: $549.00

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Product Description

The primary human pulmonary microvascular endothelial cells (HPMEC) form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. They also have metabolic properties that enable it to carry out certain important nonrespiratory function. The HPMEC are among the most important targets of reactive oxygen species elaborated in lung injury [1]. During the course of lung inflammation, neurohumoral mediators and oxidants act on endothelial cells to induce intercellular gaps permissive for transudation of proteinaceous fluid from blood into the interstitium [2]. The increased permeability leads to the hypoxemia associated with adult respiratory distress syndrome and noncardiogenic pulmonary edema [3].

HPMEC from iXCells Biotechnologies are isolated from human lung tissue. HPMEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HPMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPMEC are guaranteed to further expand for 10 population doublings in Endothelial Cell Growth Medium (Cat# MD-0010).

Product Details

  Tissue

  Human lung tissue

  Package Size

  0.5 million cells/vial  

  Passage Number

  P2

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

References

[1] Brigham, K. L. (1990) Oxidant stress and adult respiratory distress syndrome. Eur Respir J Suppl 11: 482s-484s.
[2] Moore T. M., Chetham P. M., Kelly J. J., Stevens T. (1998) Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol. 275(2 Pt 1):L203-22.
[3] Kelly JJ, Moore TM, Babal P, Diwan AH, Stevens T, Thompson WJ. (1998) Pulmonary microvascular and macrovascular endothelial cells: differential regulation of Ca2+ and permeability. Am J Physiol. 274(5 Pt 1):L810-9.

[1] Brigham, K. L. (1990) Oxidant stress and adult respiratory distress syndrome. Eur Respir J Suppl 11: 482s-484s.
[2] Moore T. M., Chetham P. M., Kelly J. J., Stevens T. (1998) Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol. 275(2 Pt 1):L203-22.
[3] Kelly JJ, Moore TM, Babal P, Diwan AH, Stevens T, Thompson WJ. (1998) Pulmonary microvascular and macrovascular endothelial cells: differential regulation of Ca2+ and permeability. Am J Physiol. 274(5 Pt 1):L810-9.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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