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Human Spleen Endothelial Cells (HSEC)

Human Spleen Endothelial Cells (HSEC)

Catalog Number: 10HU-084
Availability: Out Of Stock
Price: $679.00

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Product Description

The spleen performs essential functions in the turnover of erythrocytes. It removes erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also mounts a primary immune response to antigens in the blood and synthesizes antibodies in its white pulp. Human spleen endothelial cells (HSEC), similar to other endothelial cells, constitute the natural interface between the blood and the underlying tissue. Previous studies have demonstrated an intriguing link between splenic endothelial cells, splenic hamartoma and capillary hemangioma [1]. Splenic endothelial cells (HSEC) have also shown a supportive micro-environment for the development of dendritic cells [2]. Furthermore, rapid destruction of young erythrocytes can occur in the spleen due to altered endothelial cell-macrophage interactions [3]. These observations suggest that splenic endothelial cells may play a more compelling role in the mononuclear phagocyte system.

HSEC from iXCells Biotechnologies are isolated from human spleen. HSEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSEC are characterized by immunofluorescence with antibodies specific to VWF/Factor VIII and CD31 (PECAM), and by uptake of DiI-Ac-LDL. HSEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSEC are guaranteed to further expand for 12 population doublings in Endothelial Cell Growth Medium (Cat# MD-0010).

Product Details

  Tissue

  Human spleen tissue

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

References

[1] Zukerberg LR, Kaynor BL, Silverman ML, Harris NL. (1991) Splenic hamartoma and capillary hemangioma are distinct entities: immunohistochemical analysis of CD8 expression by endothelial cells. Hum Pathol. 22: 1258-61.
[2] Despars G, O'Neill HC. (2006) Splenic endothelial cell lines support development of dendritic cells from bone marrow. Stem Cells. 24: 1496-504.
[3] Trial J, Rice L, Alfrey CP. (2001) Erythropoietin withdrawal alters interactions between young red blood cells, splenic endothelial cells, and macrophages: an in vitro model of neocytolysis. J Investig Med. 49: 335-45.

[1] Zukerberg LR, Kaynor BL, Silverman ML, Harris NL. (1991) Splenic hamartoma and capillary hemangioma are distinct entities: immunohistochemical analysis of CD8 expression by endothelial cells. Hum Pathol. 22: 1258-61.
[2] Despars G, O'Neill HC. (2006) Splenic endothelial cell lines support development of dendritic cells from bone marrow. Stem Cells. 24: 1496-504.
[3] Trial J, Rice L, Alfrey CP. (2001) Erythropoietin withdrawal alters interactions between young red blood cells, splenic endothelial cells, and macrophages: an in vitro model of neocytolysis. J Investig Med. 49: 335-45.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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