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iXCells™ Human iPSC-Derived Myoblasts

iXCells™ Human iPSC-Derived Myoblasts

Catalog Number: 40HU-176
Availability: In Stock
Price: $1,099.00

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Product Description

iPSC-derived myoblasts are valuable tools for biochemical analysis, disease modelling.

iXCells Biotechnologies is proud to provide commercial human iPSC-derived myoblasts for differentiation into functional myotubes. iXCells™ hiPSC-derived myoblasts express typical markers, e.g. MyoD and Desmin (Figure 1) and rapidly differentiate into functional myotubes expressing markers including MHC, Dystrophin and MyoG (Figure 2), with the purity higher than 85%. Functional validation of iPSC-derived myotubes can be observed by their spontaneous twitching in the well.

iXCells™ myoblasts are available in a kit format containing both cryopreserved vials (1 or 3 million cells/vial) along with the necessary media components to expand, differentiate and maintain the myotubes in culture for at least 4-6 weeks.  Longevity of the myotubes in culture can be extended with a bi-weekly 24-hr pulse of Myoblast Expansion Medium followed by replacement with Myoblast Differentiation Medium.

iXCells also provide customized differentiation service with your own iPS cell lines.  Please contact us at orders@ixcellsbiotech.com for more details.

iPSC-Myoblasts

Figure 1.  MyoD and Desmin staining of iPSC-derived myoblasts upon recovery in Myoblast Expansion Medium.

iPSC-Myotubes

 

Figure 2.  Formation of myotubes were observed after 4 days of differentiation of iPSC-derived myoblasts in Myoblast Differentiation Medium. MHC staining of myotubes were shown by immunofluorescence staining (red).

Product Details

  Tissue

  Human iPSC-derived myoblasts (Normal, Amyotrophic Lateral Sclerosis)

  Kit Contents   Complete kit containing both cryopreserved cells along with expansion and differentiation media

  Cryopreserved

  Cell contents

  1.0 million cells/vial (Frozen)

  3.0 million cells/vial (Frozen)

  Shipped

  Cryopreserved 

  Media

  Myoblast Expansion Medium (Catalog#: MD-0102A)

  Myoblast Differentiation Medium (Catalog#: MD-0102B)

 

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This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.