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Human Cardiomyocytes (iPSC-derived, Normal)

Human Cardiomyocytes (iPSC-derived, Normal)

Catalog Number: 40HU-008
Availability: In Stock
Price: $279.00

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Product Description

Cardiomyocytes or cardiac muscle cells (also known as myocardiocytes or cardiac myocytes) are the muscle cells (myocytes) that make up the cardiac muscle.  Due to the lack of natural source of cardiac cells, in vitro differentiated human cardiomyocytes have been proven as an essential tool not only for general cardiovascular research, as well as drug development and pre-clinical research [1, 2]. 

The Comprehensive in vitro Proarrhythmie Assay (CiPA) initiative, a novel safety screening proposal led by FDA, which is intended to improve current regulatory guidances.  The new predictive technologies are evaluated, including using human stem cell-derived cardiomyocytes (hSC-CMs) in the preclinical cardiac safety assessment [3].

iXCells Biotechnologies provides ready-to-use beating human induced pluripotent stem cells (iPSC)-derived cardiomyocytes (Video 1). These cells express typical markers of cardiomyocytes, e.g. TNNT/cTnT and α-Actinin (Figure 1), with the purity higher than 90%. Functional validation of iPSC-derived cardiomyocytes has been validated by patch-clamp and Fluo-4™ Direct Calcium Assay (Video 2). When cultured with the rigorously optimized Cardiomyocyte Replating Medium (Cat# MD-0046) and Cardiomyocyte Maintenance Medium (Cat# MD-0045), the cryopreserved iXCells™ cardiomyocytes will begin beating within 3 days after thawing and become assay-ready within 10 days post replating.

All the cells provided by iXCells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C. The basic donor  information (gender / age / race) is provided for each cell lot purchased.

iXCells Biotechnologies also provides customized differentiation service.  Contact us at orders@ixcellsbiotech.com for more details. 

iPSC-derived cardiomyocyte

Figure 1.  After replating in Cardiomyocyte Maintenance Medium on Matrigel-coated plates for 2 days, more than 90% of the iPSC-derived cardiomyocytes express cTnT and α-Actinin (Figure A and B).

Video 1.  Beating human cardiomyocytes derived from iPS cells  

Video 2.  Calcium flux of iPSC-derived cardiomyocytes as shown by the Fluo-4TM Direct Calcium Assay.   

Product Details

  Tissue Origin   Human cardiomyocytes derived from iPSCs (Normal)
  Package Size   4.0 million cells/vial (frozen), enough for one 96-well plate
  Shipped   Cryopreserved
  Media

  Cardiomyocyte Replating Medium (Cat# MD-0046)

  Cardiomyocyte Maintenance Medium (Cat# MD-0045)

  Matrigel-coated Plate (Cat# MD-0023)

 

Protocols

Thawing of Frozen Cells 

  1. Upon receipt of the frozen cells, it is recommended to thaw the cells and initiate the culture immediately in order to retain the highest cell viability.
  2. To thaw the cells, put the vial in 37°C water bath with gentle agitation for ~2 minute. Keep the cap out of water to minimize the risk of contamination.
  3. Transfer the cells into a 15 mL conical tube with 5 mL Cardiomyocytes Replating Medium (Cat# MD-0046). Centrifuge at 250 g for 5 minutes at room temperature.
  4. Aspirate the supernatant, resupend the cells in 1 mL Cardiomyocytes Replating Medium. Check cell number and viability. Dilute cell to a concentration of 0.5 x 106/mL and apply 1 mL/well into matrigel-coated 24 well plate (Cat# MD-0023). Put the plate into 37°C incubator overnight.
  5. Next day, aspirate Cardiomyocytes Replating Medium, apply 1 mL Cardiomyocytes Maintenance Medium (Cat# MD-0045) to each well.
  6. Change medium with Cardiomyocytes Maintenance Medium (Cat# MD-0045) every other day.

Note:  Normally the cells will start beating within 3 days after thaw and be ready for assays within 10 days post replating.

References

[1] Matsa E, Burridge PW, Wu JC. Human stem cells for modeling heart disease and for drug discovery.Sci Transl Med. 2014 Jun 4;6(239):239ps6.
[2] Jiang W, Lan F, Zhang H. Human Induced Pluripotent Stem Cell Models of Inherited Cardiovascular Diseases. Curr Stem Cell Res Ther. 2014 Oct 16.
[3] Cavero I, Holzgrefe H. Comprehensive in vitro Proarrhythmia Assay, a novel in vitro/in silico paradigm to detect ventricular proarrhythmic liability: a visionary 21st century initiative. Expert Opin Drug Saf. 2014 Jun;13(6):745-58.

[1] Matsa E, Burridge PW, Wu JC. Human stem cells for modeling heart disease and for drug discovery.Sci Transl Med. 2014 Jun 4;6(239):239ps6.
[2] Jiang W, Lan F, Zhang H. Human Induced Pluripotent Stem Cell Models of Inherited Cardiovascular Diseases. Curr Stem Cell Res Ther. 2014 Oct 16.
[3] Cavero I, Holzgrefe H. Comprehensive in vitro Proarrhythmia Assay, a novel in vitro/in silico paradigm to detect ventricular proarrhythmic liability: a visionary 21st century initiative. Expert Opin Drug Saf. 2014 Jun;13(6):745-58.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

Related Products (2)
Name Description Catalog number
Cardiomyocyte Replating Medium MD-0046
Cardiomyocyte Maintenance Medium MD-0045