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Human iPS Cell Line (Normal)

Human iPS Cell Line (Normal)

Catalog Number: 30HU-002
Availability: In Stock
Price: $1,199.00

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Product Description

Induced Pluriopotent Stem Cells (iPSCs) are a type of pluripotent stem cells that can be derived directly from adult somatic cells [1]. The derived iPSCs can propagate indefinitely, as well as give rise to other cell types in the body.  iPS cells, thus, hold great promise in the field of regenerative medicine by representing a single source of cells that could be used to replace those damaged/diseased cells.

iXCells Biotechnologies is proud to offer human iPS cell lines derived from the human somatic cells (dermal fibroblasts, adipose-derived stem cells, peripheral blood mononuclear cells) from different race, gender, age, and tissue resource. The pertinent donor information is available upon request (info@ixcellsbiotech.com).  These iPS cells are established from a single clone and expanded in feeder-free conditions.  The Certificate of Analysis is provided for each cell lot purchased. The cells have been fully characterized for their self-renewal and pluripotency. All the cells provided by iXCells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C.     

Patient-derived iPS cell lines are also available as separate products. The currently available diseased specific iPS cell lines are derived from patients with Type 1 Diabetes (T1D), Type 2 Diabetes (T2D), Alzheimers's Disease (AD), Parkinson's Disease (PD), Amyotrophic Lateral Sclerosis (ALS)More disease-specific iPS cell lines are under development.  We also provide custom iPSC generation and iPSC differentiation services to meet your needs.

Human iPSC markers

Figure 1. iXCells human iPS cells are characterized by immunostaining with Oct4, Nanog, Sox2, SSEA4, TRA-1-60-R, TRA-1-81.

Human iPSC immunostaining

Figure 2. (A) iXCells human iPS cells are positive for the transcription factor Oct4 (green) and the surface marker SSEA4 (red). (B) iXCells human iPS cells are positive for the surface markers TRA-1-81 (green) and SSEA4 (red).

Teratoma formation

Figure 3. In vivo validation of iXCells human iPS cells by teratoma formation.

Karyotyping of iPSC Line

Figure 4. Karyotyping of iPSC Line generate by iXCells Biotechnologies.

Product Description

Tissue Origin Human iPS Cells derived from dermal fibroblasts, adipose-derived stem cells, or peripheral blood mononuclear cells
Package Size ~0.5-1.0 million cells/vial
Shipped Cryopreserved
Storage Liquid nitrogen
Growth Properties Adherent
Media & Reagents

Human iPSC Growth Medium (Cat # MD-0018)

MEF Conditioned Medium (Cat # MD-0015)

Human iPSC Feeder-Free Growth Medium (Cat # MD-0019)

Human iPSC Xeno-Free Growth Medium (Cat#0074)

iMEF Feeder (CF1), irradiated (Cat # 10MU-001)

Matrigel Coated Plates (Cat # MD-0023)

 

References

[1] Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May; 8(5):409-12.

[1] Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May; 8(5):409-12.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.