Mouse Adipose-Derived Stem Cells-white fat (MADSC-wf)
Adipose-derived stem cells (ADSC) are multipotent mesenchymal stem cells (MSC) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc in vitro. ADSC have been applied in studies including stem cell differentiation, regenerative medicine , cell therapy, tissue engineering and creation of iPS cell lines.
iXCells Biotechnologies offers mouse adipose-derived stem cells (mADSC) isolated from C57BL/6 mouse inguinal white fat tissue or interscapular brown fat tissue. These cells are cryopreserved at passage one, and can be further expanded for 3-4 passages using Adipose-derived Stem Cell Growth Medium (Cat # MD-0003). mADSC can be in vitro differentiated into adipocytes and osteoblasts (Figure 1 and 2) using Adipocyte Differentiation Medium (Cat # MD-0005) and Osteogenic Differentiation Medium (Cat # MD-0006). These cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. mADSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Figure 1. (A) Mouse ADSCs (phase contrast). (B) Adipocyte induction (Day 10 post adipogenic induction, phase contrast). (C) Adipocyte induction (Day 10 post adipogenic induction, Oil Red O staining).
Figure 2. Mouse ADSCs can be differentiated to into osteoblasts using the osteogenic induction protocol (Alizarin Red S staining, Day 21 post osteogenic induction).
|Tissue||C57BL/6 mouse inguinal white fat tissue|
|Package Size||0.5 million cells/vial|
|Media||Osteogenic Differentiation Medium (Cat # MD-0006)|
 Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.
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