Mouse stromal vascular fraction - brown fat (MSVF-bf)
Mouse stromal vascular fraction (SVF) is freshly isolated heterogeneous cell fraction derived from the brown adipose tissue (BAT, brown fat) or white adipose tissue (white fat). SVF is what remained in the pellet after removal of blood and fat components. It is crude and heterogeneous mix of multiple cell populations with different degree of maturity and function. Most sources indicate that adipose derived stem cells (ADSC) represent up to 10% (2~10%) of SVF. Endothelial cells (mature and progenitors) represents 7~30% of SVF. Fibroblasts could represent up to 50% of SVF. CD34+ cells are present at large number and could compose up to 63% of SVF.
Figure 1. Deriviation of SVF and its composition