Human Adrenal Cortical Cells (HAdCC)

Description

Product Description

The adrenal cortex makes up the perimeter of the adrenal gland and plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones^[1] . The secreted steroids arise from three zones that form the adrenal gland, which provide the framework of the adrenal cortex. The cells in the cortex stem from the mesoderm and form three concentric zones named the zona glomerulosa, zona fasciculate, and zona reticularis^[2] . Studies have shown that there is extensive interaction between the cortical and medulla regions of the adrenal gland, with cortical cells being found within the adrenal medulla and chromaffin cells within the adrenal cortex. The close contact of these two cell types implies intercellular exchange and allows for further studies concerning paracrine signaling between the two adrenal endocrine systems^[3] . Human adrenal cortex also harbours a mesenchymal stem cell-like population^[4]. Adrenal cortical cells can be used for research of hormonal regulation, steroidogenesis and regenerative therapy in adrenal insufficiency.

iXCells Biotechnologies provides high quality HAdCC, which were isolated from adult normal adrenal cortical tissue. HAdCC were cryopreserved at passage one (P1), with >0.5 million cells in each vial. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for no more than 3 passages in Adrenal Cortical Cell Growth Medium (Cat# MD-0117) under the condition suggested by iXCells Biotechnologies.

Figure 1. Phase contrast images of HAdCC. The primary cells predominantly comprised adrenocortical cells with polygonal appearance and light-refractive lipid core. Some adrenal cortical fibroblasts-like cells were also noticed in early passages. The images were taken at magnification of 10× and 20×.

Figure 2. Fluorescent immunostaining images of HAdCC. Immunocytochemistry staining was performed using antibodies against 17 beta hydroxysteroid dehydrogenase 7 (HSD17β7) and vimentin. The cells immunoreactive with HSD17β7 are steroid hormone producing cells. Nuclei were counterstained with DAPI.

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References

[1] Neelon Adrenal physiology and pharmacology. Urol Clin North Am. 1977 Jun;4(2):179-92.

[2] Pignatti, E. and Flück, CE. Adrenal cortex development and related disorders leading to adrenal insufficiency. Mol. Cell. Endocrinol. 2021 527:111-206.

[3] Schinner S, Bornstein Cortical-chromaffin cell interactions in the adrenal gland. Endocr Pathol. 2005 Summer;16(2):91-8.

[4] Gan EH, Robson W, Murphy P, Pickard R, Pearce S, Oldershaw R. Isolation of a multipotent mesenchymal stem cell-like population from human adrenal cortex. Endocr Connect. 2018 May;7(5):617-629.