Welcome visitor you can login or create an account.

Shopping Cart

0 item(s) - $0.00
Your shopping cart is empty!
Human Hepatic Stellate Cells - NASH (HHSC -N)
Human Hepatic Stellate Cells - NASH (HHSC -N)

Human Hepatic Stellate Cells - NASH (HHSC -N)

Catalog Number: 10HU-210N
Availability: In Stock
Price: $929.00

Available Options

* Package Size (Required):

Qty:     - OR -   Add to Wish List
Add to Compare

Product Description

Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCs play vital roles in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinol metabolism, storage and release. The stellate cell is the major cell type involved in liver fibrosis in response to liver injury.

In healthy liver, HSCs are in a quiescent state, and contains numerous vitamin A lipid droplets, constituting the largest reservoir of vitamin A in the body. When the liver is damaged, HSCs can change into an activated state, which is characterized by proliferation, contractility and chemotaxis. The amount of vitamin A decreases progressively in injured liver. The activated HSCs also secrete collagen scar tissue, which can lead to cirrhosis. In chronic liver disease, prolonged and repeated activation of stellate cells causes liver fibrosis [1,2].

In non-alcoholic steatohepatitis (NASH), hepatic stellate cells (HSC) differentiate into myofibroblast-like cells that cause fibrosis, which predisposes patients to cirrhosis and hepatocellular carcinoma. Thus, modeling interactions between activated HSCs and hepatocytes in vitro can aid in the development of anti-NASH/fibrosis therapeutics and lead to a better understanding of disease progression [3].

iXCells Biotechnologies provides high quality Human Hepatic Stellate Cells - Nash (HHSC -N), which are isolated from liver tissue of NASH patient, and cryopreserved with >1 million cells in each vial. Each vial is sufficient to seed on four 10 cm dishes, and cells will be ready in 5~6 days to be passaged at 1:3 ratio. HUSMC express Desmin and GFAP, and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung. HUSMC can further expand for 2-3 population doublings in Stellate Cell Growth Medium (Cat # MD-0014) under the condition suggested by iXCells Biotechnologies.

 

Product Details

  Tissue

  human liver tissue from adult NASH patient

  Package Size

  1.0 million cells/vial  

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Stellate Cell Growth Medium (Cat # MD-0014)

References

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.
[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

[3] Davidson MD. Integr Biol (Camb). 9(8):662-677.( 2017) Microengineered cultures containing human hepatic stellate cells and hepatocytes for drug development.

 

 

 

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.
[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

Related Products (1)
Name Description Catalog number
Stellate Cell Growth Medium MD-0014