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Human Osteoblasts-Calvarial (HOb-c)

Human Osteoblasts-Calvarial (HOb-c)

Catalog Number: 10HU-180
Availability: Out Of Stock
Price: $629.00

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Product Description

Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts and osteoblasts. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Osteoblasts express protease-activated receptor-1 and vascular endothelial cell growth factor [1]. Studies show that leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation [3].

iXCells Biotechnologies provides high quality Human Osteoblasts-Calvarial (HOb-c), which are isolated from human calvarial and cryopreserved at P0, with >0.5 million cells in each vial. HOb-c are characterized by the cytochemical detection of AP and mineral deposition. These HOb-c are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 10 population doublings in ​Osteoblast Growth Medium (Cat# MD-0054) under the condition suggested by iXCells Biotechnologies.

Product Details

  Tissue

  Human calvariae

  Package Size

  0.5 million cells/vial  

  Passage Number

  P0

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Osteoblast Growth Medium (Cat# MD-0054)

References

[1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. and Longaker, M. T. (2000) VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860.
[2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. (2000) Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11(6):513-20.
[3] Marie, P. J., Debiais, F., Hay, E. (2002) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. Histopathol.17(3):877-85.

[1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. and Longaker, M. T. (2000) VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860.
[2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. (2000) Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11(6):513-20.
[3] Marie, P. J., Debiais, F., Hay, E. (2002) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. Histopathol.17(3):877-85.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Osteoblast Growth Medium MD-0054