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Human Skeletal Muscle Cells (HSkMC)

Human Skeletal Muscle Cells (HSkMC)

Catalog Number: 10HU-189
Availability: Out Of Stock
Price: $619.00

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Product Description

Skeletal muscle cells, one of the largest cell types in the body, are multinucleated cells formed by the fusion of myoblasts. Skeletal muscle regeneration is a complex process. When skeletal muscle is injured, quiescent resident myoblasts called satellite cells are activated to proliferate, migrate, and differentiate [1]. Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase (MAPK) have been suggested to play an important role in skeletal muscle growth [2]. Insulin-stimulated glucose transport in cultured human skeletal muscle cells is mediated by GLUT4 and heparan sulfate proteoglycan is involved in skeletal muscle differentiation [3]. The fusion of mononucleated cells to form multinucleated myotubes is a central event in skeletal muscle development. Controlling the onset and progression of this process is a complex set of interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful model for studying the process of cell differentiation.

iXCells Biotechnologies provides high quality Human Skeletal Muscle Cells (HSkMC), which are isolated from human trapezius muscle and erector spinae muscles of the back and cryopreserved at P0, with >0.5 million cells in each vial. HSkMC express myosin, actin and actinin. These HSkMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung and can further expand for 13 population doublings in Skeletal Muscle Cell Growth Medium (Cat# MD-0052) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human trapezius muscle and erector spinae muscles of the back

  Package Size

  0.5 million cells/vial  

  Passage Number

  P0

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Skeletal Muscle Cell Growth Medium (Cat# MD-0052)

References

[1] Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78. 
[2] Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404.
[3] Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V. D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8.

[1] Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78. 
[2] Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404.
[3] Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V. D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Skeletal Muscle Cell Growth Medium MD-0052