Description
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Product Description
Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic condition, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles [1]. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100 [3].
iXCells Biotechnologies provides high quality Rat Perineurial Fibroblasts (RPNF), which are isolated from postnatal day 8 rat sciatic nerve and cryopreserved at P1, with >0.5 million cells in each vial. RPNF express vimentin, S-100, GFAP and CD90. They are negative for mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies.
Product Details
Tissue | Postnatal day 8 rat sciatic nerve |
Package Size | 0.5 millioncells/vial |
Passage Number | P1 |
Shipped | Cryopreserved |
Storage | Liquid nitrogen |
Growth Properties | Adherent |
Media | Fibroblast Growth Medium (Cat# MD-0011) |
References
[1] Salzer, J. L. (1999) Creating barriers: a new role for Schwann cells and desert hedgehog. Neuron 22:627629.
[2] Erlandson, R. A. (1991) The enigmatic perineurial cell and its participation in tumors and in tumor like entities. Ultrastruct Pathol. 15:335-351.
[3] Ariza, A., Bilbao, J. M. and Rosai, J. (1988) Immunohistochemical detection of epithelial membrane antigen in normal and perineurial cells and perineurioma. Am J Surg Pathol. 12:678-683.