30HU-101-HOM
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| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| MD-0025-2MG | 2mg (10mM in 624.4μl DMSO) | Starting at: $200.00 |
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $690.00
Product Description
Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor [1]. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency [2]. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors [3]. Y27632 in combination with Pifithrin-µ significantly improves cell recovery after cryopreservation [4].
| Catalog No. | MD-0025-2MG |
| Quantity | 2mg (10mM in 624.4μl DMSO) |
| Alternate Name | (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride |
| CAS Number | 146986-50-7 |
| Chemical Formula | C14H23Cl2N3O |
| Molecular Weight | 320.3 g/mol |
| Purity | >Greater than 98% by HPLC analysis |
| Structure |  |
| Applications | MAINTENANCE AND SELF-RENEWAL
REPROGRAMMING
DIFFERENTIATION
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| Product Use | Y27632 is for research use only. It is not approved for human or animal use, or for application in vitro diagnostic procedures. |
| Storage & stability | Store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed. |
References
[1] Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.
[2] Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.
[3] Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.
[4] Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.
[1] Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.
[2] Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.
[3] Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.
[4] Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.
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