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Human Motor Neurons (iPSC-derived, Normal)

Human Motor Neurons (iPSC-derived, Normal)

Catalog Number: 40HU-005
Availability: In Stock
Price: $1,199.00

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Product Description

Spinal motor neurons (MNs) are a highly specialized type of neurons that reside in the ventral horns and project axons to muscles to control their movement. Degeneration of MNs is implicated in a number of devastating diseases, including spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), Charcot-Marie-Tooth and poliomyelitis disease [1].  iPSC-derived motor neurons are valuable tools for biochemical analysis, disease modelling and clinical application of these diseases [2,3].

iXCells Biotechnologies is proud to provide the world’s first commercial human iPSC-derived motor neurons. iXCells™ motor neurons are derived from normal donors or ALS patientsiXCells™ motor neurons express typical markers of motor neurons, e.g. HB9 (MNX1), ISL1, CHAT (Figure 1 and Figure 2), with the purity higher than 85%. Functional validation of iPSC-derived motor neurons has been done with neuromuscular junction formation (Video 1-4).

iXCells™ motor neurons are available in both cryopreserved vials (2 million cells/vial) and fresh plate formats (12-well plate or 96-well plate). Most of the cells will express high level of HB9 and ISL-1 (Figure 1) after thawing in the Motor Neuron Maintenance Medium (Cat# MD-0022). And after cultured in the medium for 5-7 days, these cells will express high levels of CHAT and MAP2 (Figure 2). 

iXCells also provide customized differentiation service with your own iPS cell lines.  Please contact us at orders@ixcellsbiotech.com for more details.

iPSC-derived motor neurons

Figure 1.  After cultured in Motor Neuron Maintenance Medium on the Matrigel-coated plates for 2 days, more than 85% of the iPSC-derived motor neurons express HB9 (Figure A and A’),  and more than 90% of the cells express ISL1 (Figure B and B’). 

iXCells hiPSC-derived motor neurons

Figure 2.  After cultured in Motor Neuron Maintenance Medium on the Matrigel-coated plates for 5-7 days, more than 85% of the iPSC-derived motor neurons express ChAT (Figure A) and MAP2 (Figure B).

Motor neurons and astrocytes

Figure 3.  Co-culture of iXCells™ hiPSC-derived motor neurons with mouse astrocytes for 7 days. Motor neurons are stained with Tuj1, and mouse astrocytes are stained with GFAP. 

iXCells Motor Neurons Video 1. iXCellsTM human iPSC-derived motor neurons co-cultured with C2C12 mouse myotubes for 6 days.  The spontaneous contraction of myotubes was observed. 
iXCells Motor Neurons Video 2. iXCellsTM human iPSC-derived motor neurons co-cultured with C2C12 mouse myotubes for 6 days.  The contraction of myotubes was stimulated in the presence of 100µM glutamate. 
iXCells Motor Neurons Video 3. iXCellsTM human iPSC-derived motor neurons co-cultured with C2C12 mouse myotubes for 6 days.  The contraction of myotubes was inhibited by adding 100µM Curare (a neurotoxin that inhibits acetylcholine receptor at the neuromuscular junction). 

 

Video 4. iPSC-derived Motor neurons were co-cultured with C2C12 mouse myotubes for 6 days. The contraction of myotubes was monitored by calcium flux (Fluo-8).

Product Details

  Tissue

  Normal human iPSC-derived motor neurons

  Package Size

  2.0 million cells/vial (frozen)

  >5 million viable cells/plate (fresh, 96-well plate) 

  8-10 million viable cells/plate (fresh, 12-well plate)

  Shipped

  Cryopreserved or Freshly plated (12-well or 96-well plate)

  Media

  Human Motor Neuron Maintenance Medium (Cat # MD-0022)

References

[1] Brady ST. (1993). “Motor neurons and neurofilaments in sickness and in health. Cell. 9;73(1):1-3. 
[2] Dolmetsch R, Geschwind DH. (2011) “The human brain in a dish: the promise of iPSC-derived neurons”. Cell. 145(6):831-4.
[3] Payne NL, Sylvain A, O'Brien C, Herszfeld D, Sun G, Bernard CC. (2015) “Application of human induced pluripotent stem cells for modeling and treating neurodegenerative diseases.” New Biotechnology. 25;32(1):212-28.

 Download Datasheet

[1] Brady ST. (1993). “Motor neurons and neurofilaments in sickness and in health. Cell. 9;73(1):1-3. 
[2] Dolmetsch R, Geschwind DH. (2011) “The human brain in a dish: the promise of iPSC-derived neurons”. Cell. 145(6):831-4.
[3] Payne NL, Sylvain A, O'Brien C, Herszfeld D, Sun G, Bernard CC. (2015) “Application of human induced pluripotent stem cells for modeling and treating neurodegenerative diseases.” New Biotechnology. 25;32(1):212-28.

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

© iXCells Biotechnologies 2015. All rights reserved.

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Motor Neuron Maintenance Medium MD-0022