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Mouse Preadipocytes -white fat (MPAd-wf)

Mouse Preadipocytes -white fat (MPAd-wf)

Catalog Number: 10MU-106
Availability: In Stock
Price: $379.00

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Product Description

Adipose tissue is critically involved in energy storage and metabolic homeostasis of the body. Adipocyte dysfunction is involved in the pathogenesis of obesity-related insulin resistance. Adipocytes are also immunologically active and they play a role in innate immunity as well as adaptive immunity. Preadipocytes are self-renewing progenitors of mature differentiated adipocytes and can be found in adipose tissue throughout life. Previously, the majority of adipocyte research was conducted in mouse 3T3-L1 preadipocyte lines. 

iXCells Biotechnologies provides primary mouse preadipocytes (MPAd) isolated from mouse interscapular white fat tissue, which can be chemically differentiated to adipocytes in vitro (>90% efficiency). MPAd can be used as primary cell models to study adipocyte function and related metabolism and obesity research. These fibroblast-like adipocyte precursor cells can be propagated two passages in Preadipocye Growth Medium (Cat # MD-0004) prior to differentiating into mouse adipocytes (MAd).  iXCells provides optimized protocol for adipocyte differentiation.  Mature MAd are expected 7~14 days after initiation of differentiation and should remain healthy and responsive for at least 1 week after complete differentiation. MAd are ideal mouse cellular models for drug discovery research in the area of obesity, diabetics and cardiovascular diseases. 

 

Mouse preadipocytes and adipocytes

Figure 1. Mouse preadipocytes (A) and differentiated adipocytes (B and C). Mouse adipocytes (B) were differentiated 5 days from primary mouse preadipocytes (A) and cells were stained with hematoxylin and Oil-O-Red, showing lipid droplet formation in differentiated mouse adipocytes. C shows phase contrast image of MAd.

Product Details

  Tissue

  Mouse interscapular white fat

  Package Size      

  0.5x106 cells/vial  (Enough to seed on one T25 and ready to subculture in 3 days for assay or in vitro differentiation)

  Passage Number   P1
  Shipped   Frozen
  Storage           Liquid nitrogen
  Growth Properties   Adherent

References

[1]  Arianna Maiorana, Chiara Del Bianco and Stefano Cianfarani. Rev Diabet Stud (2007) 4:134-146. Adipose Tissue: A Metabolic Regulator. 
[2]  Yao, Y., M. Suraokar, B.G. Darnay, B.G. Hollier, T.E. Shaiken, T. Asano, C.-H. Chen, B.H.-J. Chang, Y. Lu, and G.B. Mills. Sci. Signal. (2013) 6(257): p. ra2. BSTA Promotes mTORC2-Mediated Phosphorylation of Akt1 to Suppress Expression of FoxC2 and Stimulate Adipocyte Differentiation. Science signaling 
[3]  Zhang, L.J., Guerrero-Juarez, C. F., Hata, T., Bapat S.P., Ramos, R., Plikus, M.V., Gallo, R.L. Science (2015) 347(6217):67-71, Dermal adipocytes protect against invasive Staphylococcus aureus skin infection
[4]  Olivia Osborn & Jerrold M Olefsky. Nature Medicine (2012), 18 (3): 363-374, The cellular and signaling networks linking the immune system and metabolism in disease

[1]  Arianna Maiorana, Chiara Del Bianco and Stefano Cianfarani. Rev Diabet Stud (2007) 4:134-146. Adipose Tissue: A Metabolic Regulator. 
[2]  Yao, Y., M. Suraokar, B.G. Darnay, B.G. Hollier, T.E. Shaiken, T. Asano, C.-H. Chen, B.H.-J. Chang, Y. Lu, and G.B. Mills. Sci. Signal. (2013) 6(257): p. ra2. BSTA Promotes mTORC2-Mediated Phosphorylation of Akt1 to Suppress Expression of FoxC2 and Stimulate Adipocyte Differentiation. Science signaling 
[3]  Zhang, L.J., Guerrero-Juarez, C. F., Hata, T., Bapat S.P., Ramos, R., Plikus, M.V., Gallo, R.L. Science (2015) 347(6217):67-71, Dermal adipocytes protect against invasive Staphylococcus aureus skin infection
[4]  Olivia Osborn & Jerrold M Olefsky. Nature Medicine (2012), 18 (3): 363-374, The cellular and signaling networks linking the immune system and metabolism in disease

This product is intended for laboratory research purposes only. It is not intended for use in humans. While iXCells Biotechnologies uses reasonable efforts to include accurate and up-to-date information on this product sheet, we make no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. iXCells Biotechnologies does not warrant that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. iXCells Biotechnologies is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, iXCells Biotechnologies is not liable for damages arising from the misidentification or misrepresentation of cultures.

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