10MU-005
Starting at:
Starting at: $449.00
Starting at: $824.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| 10MU-009 | Cryopreserved, 1.0 million cells/vial | Starting at: $824.00 |
Product Description
Mouse stromal vascular fraction (SVF) is freshly isolated heterogeneous cell fraction derived from the brown adipose tissue (BAT, brown fat) or white adipose tissue (white fat) [1]. Although not a fully defined cell population, the SVF includes vascular smooth muscle cells, fibroblasts, mast cells, macrophages, lymphocytes, endothelial cells, preadipocytes, and adipose-derived stromal/stem cells (ASCs). There is an increasing interest in the biology and therapeutic potential of SVF because of the direct and rapid isolation procedure in a xenobiotic-free environment [2].
iXCells Biotechnologies provides high quality Mouse stromal vascular fraction (SVF), which are isolated from inguinal white fat tissue (Cat# 10MU-009) or interscapular brown fat tissue (Cat# 10MU-008). The cells were cryopreserved at P0, with ≥1.0 million cells in each vial. The characterization was performed by culturing the cells using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) followed by CD29 staining (Figure 1). mSVF is negative for mycoplasma, bacteria, yeast, and fungi and can be cultured no more than 3 passages using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) under the condition suggested by iXCells Biotechnologies. Extensive expansion was not recommended because the cells may lose their multipotent properties.
Figure 1. mSVF(white fat) was recovered and cultured using Adipose-derived Stem Cells Growth Medium (Cat#MD-0003) for 7 days. (A) Phase contrast image. (B) ICC staining using CD29 antibody.
Product Details
| Tissue | C57BL/6 or BALB/C mice inguinal white fat tissue |
| Package Size | 1.0 million cells/vial |
| Passage Number | P0 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Adipose-Derived Stem Cells Growth Medium (Cat# MD-0003) |
Tan, S. S., Yeo, X. Y., Liang, Z. C., Sethi, S. K., & Tay, S. S. (2018). Stromal vascular fraction promotes fibroblast migration and cellular viability in a hyperglycemic microenvironment through up-regulation of wound healing cytokines. Experimental and Molecular Pathology, 104(3), 250-255. doi:10.1016/j.yexmp.2018.03.007 -- Learn More
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