Human Brain Microvascular Endothelial Cells (HBMVEC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10HU-051
$723.00

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Product Description

The human brain microvascular endothelial cells (HBMVEC) are the major element of the blood-brain barrier and comprise the primary limitation to passage of substances, both soluble and cellular, from the blood into the brain. HBMVEC utilize unique features that distinguish themselves from those of peripheral endothelial cells. Most prominent among these are the following: 1) intercellular ‘tight junctions’ that display high transendothelial electrical resistance and retard paracellular flux [1], 2) absence of fenestrae and a reduced level of fluid-phase endocytosis [2], and 3) asymmetrically-localized enzymes and carrier-mediated transport systems that engender a truly ‘polarized’ phenotype. Like peripheral endothelial cells, however, HBMVEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. HBMVEC has been widely used for studying the molecular and cellular basis of blood-brain barrier.

iXCells Biotechnologies provides high quality HBMVEC, which are isolated from human brain and cryopreserved at P1, with ≥ 0.5 million cells in each vial. These HBMVEC express vWF/Factor VIII, CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand no more than 3 passages in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the purity.

 

Human Brain Microvascular Endothelial

Figure 1. Human Brain Microvascular Endothelial Cells (HBMVEC). (A) Phase contrast image of HBMVEC. (B) Immunofluorescence staining with antibodies against CD31 and vWF/Factor VIII.

Product Details

  Tissue

  Human brain 

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

 

References

[1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55. 

[2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14. [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96.

Manuals & Protocols

  • Ranjan, N., Pandey, V., Panigrahi, M. K., Klumpp, L., Naumann, U., & Babu, P. P. (2021). The Tumor Suppressor MTUS1/ATIP1 Modulates Tumor Promotion in Glioma: Association with Epigenetics and DNA Repair. Cancers, 13(6), 1245. -- Learn More

  • Shima, A., Nagata, S., & Takeuchi, S. (2020). Three-dimensional co-culture of BLOOD-BRAIN BARRIER-COMPOSING cells in a culture insert with A COLLAGEN Vitrigel membrane. In Vitro Cellular & Developmental Biology - Animal, 56(7), 500-504. doi:10.1007/s11626-020-00486-x -- Learn More

  • Cui, X., Ma, C., Vasudevaraja, V., Serrano, J., Tong, J., Peng, Y., . . . Chen, W. (2020). Dissecting the immunosuppressive tumor microenvironments in glioblastoma-on-a-chip for optimized pd-1 immunotherapy. ELife, 9. doi:10.7554/elife.52253 -- Learn More

  • Heo, J., Kim, J., Woo, S., Kim, K., Choi, K., Lee, H., & Kim, K. (2019). Stromal Cell-Derived factor 1 PROTECTS Brain vascular endothelial cells FROM radiation-induced brain damage. Cells, 8(10), 1230. doi:10.3390/cells8101230 -- Learn More

  • Hunter, L. W., Jayachandran, M., & Miller, V. M. (2019). Sex differences in the expression of cell adhesion molecules on microvesicles derived from cultured human brain microvascular endothelial cells treated with inflammatory and thrombotic stimuli. Biology of Sex Differences, 10(1). doi:10.1186/s13293-019-0241-y -- Learn More

  • Ozek, C., Krolewski, R. C., Buchanan, S. M., & Rubin, L. L. (2018). Growth differentiation FACTOR 11 treatment leads to neuronal and Vascular improvements in the hippocampus of aged mice. Scientific Reports, 8(1). doi:10.1038/s41598-018-35716-6 -- Learn More

Biological
Cell System Nervous Cell System
Cell System Vascular Cell System
Cell Type Endothelial Cells
Species Human (Normal)