Starting at: $812.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| 10HU-133 | Cryopreserved, 0.5 million cells/vial | Starting at: $812.00 |
Product Description
Synovial joints are unique functional elements of the body and provide the ability for locomotion and for physical interaction with the environment. The inner lining of the joint consists of the synovium, also called the synovial membrane, a thin layer located between the joint capsule and the joint cavity. Fibroblast-like synoviocytes (FLS) are mesenchymal origin cells located inside the synovial membrane. FLS display many characteristics common with fibroblasts, such as expression of several types of collagens and protein vimentin, a part of cytoskeletal filament [1]. Once activated, FLS are suggested to acquire a myofibroblast-like phenotype that drives fibrogenesis through excessive extracellular matrix component deposition and an enhanced contractile function [2]. These cells play a crucial role in the pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis [1, 3]. Therefore, Human FLS are an important model to understand articular function in degenerative and inflammatory joint diseases.
iXCells Biotechnologies provides high quality Human Fibroblast-Like Synoviocytes (HFLS), which are isolated from human synovial membrane and cryopreserved at P2, with ≥0.5 million cells in each vial. HFLS express fibronectin and vimentin and are characterized by their spindle-shaped morphology. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages in Synoviocyte Growth Medium (Cat# MD-0056) under the conditions suggested by iXCells Biotechnologies.
Figure 1. Phase contrast and Immunofluorescence staining with antibodies against Vimentin and Fibronectin of Human Fibroblast-Like Synoviocytes(HFLS).
Product Details
| Tissue | Human Synovial Membrane |
| Package Size | 0.5 million cells/vial |
| Passage Number | P2 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Synoviocyte Growth Medium (Cat# MD-0056) |
References
[1] Pap, T., Dankbar, B., Wehmeyer, C., Korb-Pap, A., & Sherwood, J. (2020). Synovial fibroblasts and articular tissue remodelling: Role and mechanisms. Seminars in cell & developmental biology, 101, 140–145.
[2] Maglaviceanu, A., Wu, B., & Kapoor, M. (2021). Fibroblast-like synoviocytes: Role in synovial fibrosis associated with osteoarthritis. Wound repair and regeneration; official publication of the Wound Healing Society [and] the European Tissue Repair Society, 29(4), 642–649.
[3] Chang, S. K., Gu, Z., & Brenner, M. B. (2010). Fibroblast-like synoviocytes in inflammatory arthritis pathology: the emerging role of cadherin-11. Immunological reviews, 233(1), 256–266.
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