Human Hepatic Sinusoidal Endothelial Cells (HHSEC, Adult)

Product Description

Human liver contains two distinct endothelial cell types: vascular and sinusoidal. Human Hepatic Sinusoidal Endothelial Cells (HHSEC) are microvascular endothelial cells that exhibit a unique phenotype compared to other endothelial cells—they function as dendritic cells to present antigen for CD4+ T cells. Thus, HHSEC are actively involved in the regulation of the immune response in the liver ^[1]. HHSEC are actively involved in liver repair as dynamic regulators respond rapidly and locally to environmental zonal stimuli ^[2].

iXCells Biotechnologies provides high quality HHSEC, which are isolated from normal human liver and cryopreserved at P2, with >0.5 million cells in each vial. HHSEC express vWF/Factor VIII and CD31 (Figure 2). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Endothelial Cell Growth Medium (Cat# MD-0010) for no more than 3 passages under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the cell purity.

Figure 1.  Human Hepatic Sinusoidal endothelial cells (HHSECs) (phase contrast).

Figure 2.  HHSECs provided by iXCells are positive for CD31 (A) and vWF (B), as shown by immunostaining. Flow analysis showed that more than 98% of cells are CD31 positive (A'), and more than 99% are vWF positive (B').            

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References

[1]  Limmer A, and Knolle PA. Liver sinusoidal endothelial cells: a new type of organ-resident antigen-presenting cell. Arch Immunol Ther Exp (Warsz) 2001; 49 (1): S7-11.

[2]  Wach KE etc. Sinusoidal ultrastructure evaluated during the revascularization of regenerating rat liver. Hepatoloty 2001; 33 (2): 363-378.