Human Pancreatic Microvascular Endothelial Cells (HPaMEC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10HU-071
$593.00

 

Product Description

The pancreas is an endocrine gland and digestive organ which secretes hormones and produces pancreatic juice to aid in digestion[1,2].Pancreatic islets function as pancreatic endocrine cells to produce vital hormones such as glucagon, insulin, and amylin[1,2]. Hence, Human Pancreatic Microvascular Endothelial Cells (HPaMEC) play a critical role to support the islet by transporting oxygen and nutrients to the pancreas, and affecting beta ell function and proliferation, influencing insulin gene expression during islet development, and product various growth factors[1,2]. Additionally, HPaMEC are involved in optimizing blood glucose sensing and regulation. HPaMEC can be used an in vitro model for studying islet biology, pancreatic cancer, transplantation, and regenerative medicine.

iXCells Biotechnologies provides high quality HPaMEC, which are isolated from human pancreatic tissue and cryopreserved at P2 after purification, with ≥0.5 million cells in each vial. HPaMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII, VE-Cadherin and/or CD31 (PECAM) (Figure 1). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPaMEC can proliferate in Endothelial Cell Growth Medium (Cat# MD-0010), but they are not recommended for further expansion, because the purity of the endothelial population may decrease.

10HU 071 HPaMEC

Figure 1. Human Pancreatic Microvascular Endothelial Cells (HPaMEC). (A) Phase contrast image of HPaMEC. (B & C) Immunofluorescence staining with antibodies against VE-Cadherin (B) and vWF/Factor VIII (C).

 

Product Details

  Tissue

  Human pancreatic tissue

  Package Size

  0.5 x 10^6 cells/vial

  Passage Number

  P2

  Shipped

  Cryopreserved

  Storage

  Liquid Nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

 

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Reference

[1] Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9.[1] Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9.

[2] Konstantinova I and Lammert E. (2004). “Microvascular development: learning from pancreatic islets.” Bioessays, 26(10):1069-1075.

[1] Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9.[1] Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9.

[2] Konstantinova I and Lammert E. (2004). “Microvascular development: learning from pancreatic islets.” Bioessays, 26(10):1069-1075.

Biological
Cell System Endocrine Cell System
Cell System Vascular Cell System
Cell Type Endothelial Cells
Species Human (Normal)