Human Peripheral Blood CD14+ Monocytes (Positive Selection)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 10 million cells/vial
10HU-008-10M
$699.00
Cryopreserved, 40 million cells/vial
10HU-008-40M
$1,279.00

Product Description

Monocytes are innate blood cells that maintain vascular homeostasis and are early responders to pathogens in acute infections [1, 2].  Monocytes constitute 10~30% of peripheral blood mononuclear cells in the human body. They play multiple roles in immune function including replenishing resident macrophages under normal states. And in response to inflammation signals, monocytes can move quickly (approx. 8–12 hours) to sites of infection in the tissues and divide/differentiate into macrophages and dendritic cells to elicit an immune response [3]. CD14 is an important surface marker for monocytes.

iXCells Biotechnologies offers CD14+ monocytes isolated from normal human peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic selection.  > 90% of the cells are CD14+ as showed by flow cytometric analysis (Figure 1 A). And >53% of monocytes are capable to differentiate into mature dendritic cell (Fig 1B, C).

CD14+ monocytes

Figure 1. Human Peripheral Blood CD14+ Monocytes (Positive Selection). (A) >90% of purified cells are CD14 positive. (B, C) Flow cytometric analysis of monocytes dendritic cell differentiation, showed >53% of cells are CD83 positive and >77% cells are CD1a positive.

Product Details

  Tissue

  Normal human peripheral blood

  Package Size

  1.0x107 cells/vial, 4.0x107 cells/vial

  Purity

  >90%

  Passage Number

  P0

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Suspension

  Media

  Blood Cell Culture Medium (Cat# MD-0007)

 

References

[1] Martin Guilliams, Alexander Mildner, and Simon Yona. (2018) “Developmental and Functional Heterogeneity of Monocytes”. Immunity, 49: 595-613.

[2] Prakash Babu Narasimhan, Paola Marcovecchio, Anouk A.J. Hamers, and Catherine C. Hedrick. (2019) “Nonclassical Monocytes in Health and Disease”. Annual Reviews of Immunology, 37: 439-456.

[3] Ziegler-Heitbrock, L (2007). "The CD14+ CD16+ Blood Monocytes: their Role in Infection and Inflammation, Review". J Leukocyte Biology 81 (3): 584–92.

 

 Download Datasheet

[1] Martin Guilliams, Alexander Mildner, and Simon Yona. (2018) “Developmental and Functional Heterogeneity of Monocytes”. Immunity, 49: 595-613.

[2] Prakash Babu Narasimhan, Paola Marcovecchio, Anouk A.J. Hamers, and Catherine C. Hedrick. (2019) “Nonclassical Monocytes in Health and Disease”. Annual Reviews of Immunology, 37: 439-456.

[3] Ziegler-Heitbrock, L (2007). "The CD14+ CD16+ Blood Monocytes: their Role in Infection and Inflammation, Review". J Leukocyte Biology 81 (3): 584–92.

Biological
Cell System Hematopoietic Cell System
Cell Type Immune Cells AND Hematopoietic Cells
Species Human (Normal)