Human Prostate Epithelial Cells (HPrEpC)

Product Description

The prostate is a major accessory organ of the male mammalian reproductive system, with structural and functional activities under the control of androgens. The normal prostate is composed of three compartments, which can be identified by the cytokeratin (CK) expression profile ^[1]. CK5 and -14 are prominently expressed in the basal cells whereas, the secretory compartment consisting of luminal layer of columnar cells, constitutively express CK8 and CK18. On the other hand, the intermediate cells express a combination of basal and luminal cytokeratins ^[2,3]. Additionally, there is increasing evidence that basal epithelial cells can differentiate into luminal epithelial cells. Prostate cancer is one of the most common cancers affecting men and it has a high morbidity and mortality rate. Human primary prostate epithelial cell (HPrEpC) culture provides unique opportunities to study many important features of the prostate, as well as chemical and hormonal carcinogenesis.

iXCells Biotechnologies provides high quality HPrEpC, which are isolated from normal human prostate tissue and cryopreserved at P1, with >0.5 million cells in each vial. HPrEpC express cytokeratin-14, -18 and -19. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 12 population doublings in Epithelial Cell Growth Medium (Cat# MD-0041) under the condition suggested by iXCells Biotechnologies.

Figure 1: Phase contrast and cytokeratin 14 (C K 14) staining post recovery

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Reference

[1] van Leenders G, Dijkman H, Hulsbergen-van de Kaa C, Ruiter D, Schalken J: Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab Invest 2000, 80:1251-1258.

[2] Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C: Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 1990, 143:167-171

[3] De Marzo, A. M., Meeker, A. K., Epstein, J. I., & Coffey, D. S. (1998). Prostate stem cell compartments: expression of the cell cycle inhibitor p27Kip1 in normal, hyperplastic, and neoplastic cells. The American journal of pathology, 153(3), 911–919.