Human Skeletal Muscle Myoblasts (HSkMM)

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10HU-176

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Product Description

Skeletal muscle regenerates by the proliferation of mononuclear myogenic precursor cells called myoblasts that ultimately fuse and become incorporated into multinucleated myotubes. The myotubes then later mature into myofibers. This process occurs during the embryonic histogenesis of muscle, in postnatal muscle regeneration in response to injury, or in diseases such as Duchenne muscular dystrophy. The fusion of myoblasts is specific to skeletal muscle and myoblasts that do not form muscle fibers differentiate into satellite cells. Skeletal muscle myoblasts express the FGF receptor [1] and IGF expression is increased during myoblast differentiation in culture [2, 3]. The human skeletal muscle myoblast culture is a convenient in vitro model for the study of cellular development and differentiation, insulin metabolism and tissue repair.

iXCells Biotechnologies provides high quality Human Skeletal Muscle Myoblasts (HSkMM), which are isolated from human muscle of the pectoral girdle and cryopreserved at P1, with >0.5 million cells in each vial. HSkMM express myosin, actin and actinin. These HSkMM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 12 population doublings in Skeletal Muscle Cell Growth Medium (Cat# MD-0052) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human muscle of the pectoral girdle

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Skeletal Muscle Cell Growth Medium (Cat# MD-0052)

References

[1]. Olwin BB, Hauschka SD. (1986) Identification of the fibroblast growth factor receptor of Swiss 3T3 cells and mouse skeletal muscle myoblasts. Biochemistry 25(12):3487-92. 
[2]. Tollefsen S, Lajara R, McCusker RH, Clemmons DR, Rotwein P (1989) Insulin-like growth factors in muscle development. J Biol Chem 264:13810-13817. 
[3]. Kou Kou, Rotwein PS (1993) Transcriptional activation of the insulin-like growth factor-II gene during myoblast differentiation. Mol Endocrinol 7:291-302.

[1]. Olwin BB, Hauschka SD. (1986) Identification of the fibroblast growth factor receptor of Swiss 3T3 cells and mouse skeletal muscle myoblasts. Biochemistry 25(12):3487-92. 
[2]. Tollefsen S, Lajara R, McCusker RH, Clemmons DR, Rotwein P (1989) Insulin-like growth factors in muscle development. J Biol Chem 264:13810-13817. 
[3]. Kou Kou, Rotwein PS (1993) Transcriptional activation of the insulin-like growth factor-II gene during myoblast differentiation. Mol Endocrinol 7:291-302.

Biological
Cell System Skeletal Muscle Cell System
Cell Type Muscle Myoblasts
Species Human (Normal)