Human Umbilical Mesenchymal Stem Cells (HUMSC)

Product Description

Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine. MSC isolated from the Wharton’s jelly of the umbilical cord were induced to transform into neurons and glia in vitro by using neuron-conditioned medium, sonic hedgehog, and FGF-8 ^{[1, 2]}. MSC can also differentiate into cells from the adipogenic and osteogenic lineage. Additionally, they have the potential to differentiate into cardiomyocytes by culturing them in cardiomyocyte-conditioned medium ^[3]. MSC express the matrix receptors CD44 and CD105 and mesenchymal stem cell markers SH2 and SH3, but not the hematopoietic lineage marker CD34.

Human Umbilical Mesenchymal Stem Cells (HUMSC) from iXCells Biotechnologies are isolated from Wharton’s jelly of the umbilical cord. HUMSC are cryopreserved at passage one and delivered frozen. Each vial contains 0.5 million cells in 1 ml volume. HUMSC are characterized by immunofluorescence with antibodies specific to CD73, CD90 and CD105. HUMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUMSC can expand for no more than 3 passages under the conditions recommended by iXCells Biotechnologies using Mesenchymal Stem Cell Medium (Cat# MD-0037).

Figure 1.  Human Umbilical Mesenchymal Stem Cells (HUMSC). (A) Phase contrast image of HUMSC. (B) Immunofluorescence staining with antibodies against CD29 and CD90.

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References

[1] Mitchell, K. E. et al. (2003) Matrix cells from Wharton's jelly form neurons and glia. Stem Cells 21:50-60. 

[2] Fu, Y. S. et al. (2006) Conversion of human umbilical cord mesenchymal stem cells in Wharton's jelly to dopaminergic neurons in vitro: potential therapeutic application for Parkinsonium. Stem cells 24:115-124. 

[3] Wang, H. S., et al., (2004) Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem cells 22:1330-1337.