Human Umbilical Vein Endothelial Cells (HUVEC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10HU-012
$399.00

Product Description

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis) [1]. They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth [2]. HUVECs can be easily made to proliferate in a laboratory setting. They exhibit a cobblestone phenotype when lining vessel walls.

iXCells Biotechnologies provides high quality Human Umbilical Vein Endothelial Cells (HUVEC), which are isolated from human umbilical vein of mix donors, and cryopreserved at P2, with >0.5 million cells in each vial. HUVEC have “cobblestone” morphology and positive staining with vWF/Factor VIII and CD31. HUVEC are one of the mostly used cell types to study endothelial function in vitro, including angiogenesis [1], signaling pathway under normal and pathological condition such as oxidative stress, hypoxia [2] and inflammation etc. These HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi and can further expand no more than 3 passages in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies. Additional expansion may decrease the purity.

 

Human Umbilical Vein Endothelial Cells

Figure 1. Human Umbilical Vein Endothelial Cells (HUVEC). (A) Phase contrast image of HUVEC. (B & C) Immunofluorescence staining with antibodies against vWF (B) and CD31 (C).

 

Product Details

  Tissue

  Human umbilical cord tissue

  Package Size

  0.5x106 cells/vial  

  Passage Number

  P2

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

 

References

[1]  Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB (2006). "Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis". Stem Cell Rev 2 (2): 93–102.

[2]  Nallamshetty S, Chan SY, and Loscalzo J. Hypoxia: a master regulator of microRNA biogenesis and activity. Free Radic Biol Med. 2013; 64: 20-30.

 

 Download Datasheet

[1]  Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB (2006). "Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis". Stem Cell Rev 2 (2): 93–102.

[2]  Nallamshetty S, Chan SY, and Loscalzo J. Hypoxia: a master regulator of microRNA biogenesis and activity. Free Radic Biol Med. 2013; 64: 20-30.

Biological
Cell System Umbilical Cord Cell System
Cell System Vascular Cell System
Cell Type Endothelial Cells
Species Human (Normal)