Human Hepatic Stellate Cells- Adult (HHSC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 1.0 million cells/vial
10HU-210
$929.00

Product Description

Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCs play vital roles in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinol metabolism, storage and release. The stellate cell is the major cell type involved in liver fibrosis in response to liver injury. In healthy liver, HSCs are in a quiescent state, and contains numerous vitamin A lipid droplets, constituting the largest reservoir of vitamin A in the body. When the liver is damaged, HSCs can change into an activated state, which is characterized by proliferation, contractility and chemotaxis. The amount of vitamin A decreases progressively in injured liver. The activated HSCs also secrete collagen scar tissue, which can lead to cirrhosis. In chronic liver disease, prolonged and repeated activation of stellate cells causes liver fibrosis [1,2]. Primary culture of HSC is a valuable tool to study liver fibrosis.

Human Hepatic Stellate Cells (HHSC) from iXCells Biotechnologies are isolated from adult human liver and cryopreserved with > 1 million cells in each vial. Each vial is enough to seed on four 10 cm dishes, and cells will be ready in 5~6 days to be passaged at 1:3 ratio. HHSC are characterized by immunofluorescence with antibodies specific to Desmin and GFAP. HHSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHSC can be further expanded for 2-3 passages in Stellate Cell Growth Medium (Cat# MD-0014).

 

Human Hepatic Stellate Cells

Figure 1. Human hepatic stellate cells (HHSC) were stained with HSC markers, including Desmin and GFPA as indicated (green).  Phase contrast images of each staining is shown on the right panel.  

 

Product Details

  Tissue

  Adult human liver

  Package Size

  1.0 million cells/vial  

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Stellate Cell Growth Medium (Cat # MD-0014)

 

References

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.

[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

 

 
 

* Holiday discount is not applicable to this product *

 

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.

[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

Biological
Cell System Hepatic Cell System
Cell Type Stellate Cells
Species Human (Normal)