Human Hepatic Stellate Cells- Adult (HHSC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 1.0 million cells/vial
10HU-210
$1,004.00

Product Description

Hepatic stellate cells (HSC) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSC play vital roles in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinol metabolism, storage, and release.  Stellate cell is the major cell type involved in liver fibrosis in response to liver injury. In healthy liver, HSC are in a quiescent state, and contains numerous vitamin A lipid droplets, constituting the largest reservoir of vitamin A in the body. When the liver is damaged, HSC can change into an activated state, which is characterized by proliferation, contractility, and chemotaxis. The amount of vitamin A decreases progressively in injured liver. The activated HSC is also responsible for secreting collagen scar tissue, which can lead to cirrhosis. In chronic liver disease, prolonged and repeated activation of stellate cells causes liver fibrosis [1,2]. Primary culture of HSC is a valuable tool to study liver fibrosis.

Human Hepatic Stellate Cells (HHSC) from iXCells Biotechnologies are isolated from adult human liver and cryopreserved with ≥ 1 million cells in each vial. HHSC are characterized by immunofluorescence with antibodies specific to Desmin and GFAP. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HHSC can be further expanded for 2-3 passages in iXCells’ Stellate Cell Growth Medium (Cat# MD-0014).

 

10HU 210 Figure

Figure 1. Human hepatic stellate cells (HHSC) stained with Desmin and GFAP (green).  Corresponding phase contrast images of each staining is shown to the right.   

 

Product Details

  Tissue

  Adult human liver

  Package Size

  1.0 million cells/vial  

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Stellate Cell Growth Medium (Cat # MD-0014)

 

References

[1] Yin, C., Evason, K. J., Asahina, K., & Stainier, D. Y. (2013). Hepatic stellate cells in liver development, regeneration, and cancer. The Journal of clinical investigation, 123(5), 1902–1910.

[2] Rockey D. C. (2001). Hepatic blood flow regulation by stellate cells in normal and injured liver. Seminars in liver disease, 21(3), 337–349.

 

 
 

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  • Kim, J. H., Lee, C. H., & Lee, S. (2019). Exosomal transmission of microrna from hcv replicating cells stimulates transdifferentiation in hepatic stellate cells. Molecular Therapy - Nucleic Acids, 14, 483-497. doi:10.1016/j.omtn.2019.01.006 -- Learn More

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Biological
Cell System Hepatic Cell System
Cell Type Stellate Cells
Species Human (Normal)

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