Human Cortical GABAergic Neurons (iPSC-derived, Normal)

Product Description

Excitation and inhibition are the two basic interactions between neurons, which utilize glutamate and γ-aminobutyric acid (GABA) as the major neurotransmitters for excitatory and inhibitory signals, respectively. Abnormal GABAergic neuron function has been associated with multiple neurodevelopmental and neurodegenerative disorders.

iXCells™ Cortical GABAergic Neurons show high neuronal purity (>90% Tuj1 positive cells) and express typical marker GABA (Figure 1 and Figure 2) after culturing in the Cortical Neuron Maintenance Medium (Cat# MD-0093) for 5 days.

iXCells also provide customized differentiation service with your own iPS cell lines.  Please contact us at This email address is being protected from spambots. You need JavaScript enabled to view it. for more details.

Figure 1.  Phase contrast image of iPSC-GABAergic neurons cultured in the Cortical Neuron Maintenance Medium (Cat# MD-0093) for 5 days. 

Figure 2.  The cryopreserved GABAergic neurons were recovered and seeded on Matrigel-coated 96-well plate and cultured in the Cortical Neuron Maintenance Medium (Cat# MD-0093) for 5 days. The cells were fixed and stained with anti-GABA (red) and anti-TUJ1 (green) antibodies.

Product Details

References

[1] Yang N, Chanda S et al. (2017). “Generation of pure GABAergic neurons by transcription factor programming”. Nat Methods. 14(6):621-628. 

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