Human Skeletal Muscle Myoblasts (iPSC-derived, Normal)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 1.0 million cells/vial
40HU-176-1M
$1,187.00
Cryopreserved, 3.0 million cells/vial
40HU-176-3M
$2,699.00

Product Description

iPSC-derived skeletal muscle myoblasts are valuable tools for biochemical analysis, disease modelling.

iXCells Biotechnologies is proud to provide ready-to-use human iPSC-derived skeletal muscle myoblasts for differentiation into functional myotubes. iXCells™ hiPSC-derived myoblasts express typical markers, e.g. MyoD and Desmin (Figure 1) and rapidly differentiate into functional myotubes expressing markers including MHC, Dystrophin and MyoG (Figure 2), with the purity higher than 85%. Functional validation of iPSC-derived myotubes can be observed by their spontaneous twitching in the well. iXCells™ human iPSC-derived skeletal muscle myoblasts are available in a kit format containing both cryopreserved vials (1 or 3 million cells/vial) along with the necessary media components to expand, differentiate and maintain the myotubes in culture for at least 4-6 weeks. Longevity of the myotubes in culture can be extended with a bi-weekly 24-hr pulse of Myoblast Expansion Medium (Cat# MD-0102A) followed by replacement with Myoblast Differentiation Medium (Cat# MD-0102B).

iXCells also provide customized differentiation service with your own iPS cell lines.  Please contact us at This email address is being protected from spambots. You need JavaScript enabled to view it. for more details.

iPSC-myoblasts_Fig_1

Figure 1.  MyoD and Desmin staining of human iPSC-derived myoblasts upon recovery in the Myoblast Expansion Medium (Cat# MD-0102A).

 

Myotubes

Figure 2.  Formation of myotubes were observed after 4 days of differentiation of human iPSC-derived myoblasts in the Myoblast Differentiation Medium (Cat# MD-0102B). MHC staining of myotubes were shown by immunofluorescence staining (red).

 

Product Details

  Tissue Origin

  Human iPSC-derived myoblasts (Normal, ALS)

  Kit Contents   Complete kit containing both cryopreserved cells along with expansion and differentiation media

  Cryopreserved Cell   contents

  1.0 million cells/vial (Frozen)
  3.0 million cells/vial (Frozen)

  Shipped

  Cryopreserved 

  Media

  iPSC-Derived Myoblast Expansion Medium (Cat# MD-0102A)
  iPSC-Derved Myoblast Differentiation Medium (Cat# MD-0102B)

 

Download Datasheet

 

 

Biological
Species Human (Normal)