Genome Editing Services
iXCells offers complete genome editing services according to your specifications to help you accelerate your research. Our experts have extensive experience in various types of gene editing including a SNP replacement, large reporter gene knock-in and pieces of DNA knock-out in iPSC and other cell lines using CRISPR/Cas9 technology. It can be done with in-house iPSC lines or custom-provided cell lines. For cell lines derived from patients, they need to have been tested to be negative for Mycoplasma, HIV, Hepatitis B and C. The service takes from 2 to 5 months to complete including delivery of 2-3 clones, which are sequence confirmed for right editing. We can keep you timely updated and our scientists are always ready when you have questions during the process. The final project report will be provided when the study is completed.
iXCells GE Service Capabilities
Types of Gene Editing
- CRISPR/Cas9
- Zinc finger
- TALEN
Types of Cells
- Stem cells
- Non-stem cell lines
Types of Services
- Knock-in
- Reporters, exon duplication, overexpression, safe harvest sites etc.
- Knock-out
- Frameshift, stop codon, fragment deletion
- Point mutation
- Single nucleotide mutation, small fragment replacement
Special Designs for KI and KO
Knock-in
- KI of NGN2 at genomic safe harbor sites
- Duplication of Exons
- Luciferase, fluorescence marker and label tags KI for different genes
Knock-out
- KO by frame shift
- KO by introducing stop codon
- KO by large fragment deletion
Why Choose Us
01
Expertise in iPSC Culture
02
High Efficiency Genome Editing
RNP Transfection
- Time effective
- High efficiency
- No need for plasmid
HDR Enhancer
- Increase HDR efficiency for identifying KI clones
03
High Efficiency for Single Clone Generation
Single Cell Picker
- Increase single cell survival
- Easy single clone generation
- No cell damaging by sorting
- Less labor intensity
04
KI Sequence Confirmed by Sanger Sequencing
Genome Editing Service Workflow:
Frequently asked questions (FAQs)
What information should I provide to get a quote?
You will need to fill in the questionnaire (Link) form. Briefly you will need to provide the cells to be edited, the Gene name, KI/the insertion/deletion locus if specified, and the validation methods if specified.
What do I need to supply for this service?
How long is the turnaround time?
What are the deliverables?
What do you provide as technical support?
