You will need to fill in the questionnaire (Link) form. Briefly you will need to provide the cells to be edited, the Gene name & accession number, the genomic sequence before/after editing, the insertion/deletion locus if specified, and the validation methods if specified.

• Cells to be edited: Two vials of frozen stocks on dry ice or one T25 flask of live cells per sample. The cells have to be actively dividing cells. Non-dividing cells or slow-proliferating cells are not able to be edited. iXCells also provides a wide collection of primary cells/iPSCs (link) for the customers to choose from.
• Recipe of the culture media.
• Culture protocol.
• Pathogen screening data.
• Transfection protocol if available.

It takes 2-4 months depending on the recovery of the cells provided by the customer, the proliferation rate and transfection efficiency of the cells, and validation assays requested by the customer.

 We will ship 3 frozen vials of each clone to the customer. Live cultures are available upon requests. We will also provide characterization data in publication-ready format.

 We will provide 1) the gene editing method we used to edit the cell line; 2) the recovery and maintenance method after you receive the cells; 3) Cell recovery and live culture delivery if desired; 4) in-house training course if desired.