Human Dopaminergic Neurons (iPSC-derived)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 2.0 million cells/vial
40HU-012
$1,349.00

Product Description

Dopaminergic (DA) neurons are the major cells generating a neurotransmitter, dopamine, in the central nervous system. DA neurons reside in various regions extending from the midbrain to the forebrain in mammals. Loss of DA neurons in the substantia nigra pars compacta (SNc; AKA A9 group) in the midbrain region may contribute to the motor disturbance in Parkinson’s disease (PD)1. However, what causes DA neuron to degenerate or die is still unknow1. To understand the PD progression and develop effective therapeutic approaches in laboratories, a clinical relevant and cell-based platform is needed1,2,3. Therefore, human induced pluripotent stem cell (iPSC) derived DA neurons can serve as a powerful tool to investigate the underlying molecular mechanisms, to model AD in vitro for drug discovery, and to pave the road for cell therapy development4.Dopaminergic (DA) neurons are the major cells generating a neurotransmitter, dopamine, in the central nervous system. DA neurons reside in various regions extending from the midbrain to the forebrain in mammals. Loss of DA neurons in the substantia nigra pars compacta (SNc; AKA A9 group) in the midbrain region may contribute to the motor disturbance in Parkinson’s disease (PD)1. However, what causes DA neuron to degenerate or die is still unknow1. To understand the PD progression and develop effective therapeutic approaches in laboratories, a clinical relevant and cell-based platform is needed1,2,3. Therefore, human induced pluripotent stem cell (iPSC) derived DA neurons can serve as a powerful tool to investigate the underlying molecular mechanisms, to model AD in vitro for drug discovery, and to pave the road for cell therapy development4.

iXCells Biotechnologies is excited to provide DA neuron-like cells derived from human iPSCs. Our hiPSC-derived DA neurons express typical DA neuron marker, tyrosine hydroxylase (TH), as well as mature neuron marker microtubule associated protein 2 (MAP2) (Figure 1).iXCells DA neurons are available in cryopreserved vials (2 x 106 cells/vial). The cells can be recovered in the DA Neuron Maintenance Medium. And after 10-14 days of recovery, these cells will mature into neuron-like cells and will express high levels of TH and dopamine transporter (DAT) (Figure 1, 2, and 3).

 

human-dopaminergic-neurons

Figure 1. Mature DA neuron-like cells derived from human iPSCs highly express MAP2 and TH markers.  (A) Cells were recovered and cultured for 4 days in DA Neuron Maintenance Medium. Robust neurogenesis was observed. (B) Cells werefurther cultured and matured in DA Neuron Maturation Medium. (C) to (F) Representative images show that DA neuronsexpress MAP2 and TH. Cells were recovered from cryovial and cultured for 11 days. Identity of DA neurons was confirmedby immunostaining using MAP2 and TH antibodies. More than 85% of the human iPSC-derived DA neurons are TH positive(estimated by immunostaining). Nuclei were stained with DAPI.

 

human-dopaminergic-neurons

Figure 2. Many TUJ1+ neuron-like cells express DAT. Cells were cultured for 2 weeks and used to perform immunostaining of TUJ1 and DAT. More than 50% of the human iPSC-derived DA neurons are DAT positive (estimated by immunostaining). Nuclei were stained with DAPI.

 

human-dopaminergic-neurons

Figure 3. Majority of mature neurons express tyrosine hydroxylase. Cells were cultured for 2 weeksFigure 3. Majority of mature neurons express tyrosine hydroxylase. Cells were cultured for 2 weeksand used to perform immunostaining of TH. TH signal was detected and quantified by flow cytometryanalysis. More than 80% of the human iPSC-derived DA neurons are TH positive. Non-staining cellsserve as negative control (Control).

 

Product Details

  Tissue Origin

  Human iPSC-derived Dopaminergic Neurons (Normal)

  Package Size

 2 x 106 cells/vial (frozen)

  Shipped

 Cryopreserved

  Media

 Human Dopaminergic Neuron Maintenance Medium (Cat# MD-0105A)

 Human Dopaminergic Neuron Maturation Medium (Cat# MD-0105B)

 

 Download Datasheet

[1] Julie Lotharius and Patrik Brundin. “Pathogenesis of Parkinson’s disease: dopamine, vesicles and a-synuclein” Nat Rev Neurosci 2002 3(12):932-42.

[2] Ernest Arenas, Mark Denham, J. Carlos Villaescusa. “How to make a midbrain dopaminergic neuron” Development 2015 142:1918-1936.

[3] Rodolfo Gonzalez, et al., “Deriving dopaminergic neurons for clinical use. A practical approach” Scientific Reports 2013 3:1463-1467.

[4] Curt R. Reed, et al., “Transplantation of Embryonic Dopamine Neurons for Severe Parkinson's disease” N Engl J Med 2001 344:710-719.

Biological
Species Human (Normal)