Human Motor Neurons (iPSC-derived, Normal)
Spinal motor neurons (MNs) are a highly specialized type of neurons that reside in the ventral horns and project axons to muscles to control their movement. Degeneration of MNs is implicated in a number of devastating diseases, including spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), Charcot-Marie-Tooth and poliomyelitis disease. iPSC-derived motor neurons are valuable tools for biochemical analysis, disease modelling and clinical application of these diseases [2,3].
iXCells Biotechnologies is proud to provide the world’s first commercial human iPSC-derived motor neurons. iXCells™ hiPSC-derived motor neurons express typical markers of motor neurons, e.g. HB9 (MNX1), ISL1, CHAT (Figure 1 and Figure 2), with the purity higher than 85%. Functional validation of iPSC-derived motor neurons has been done with neuromuscular junction formation.
XCells™ motor neurons are available in both cryopreserved vials (1 to 4 million cells/vial) .Most of the cells will express high level of HB9 and ISL-1 (Figure 1) after thawing in the Motor Neuron Maintenance Medium (Cat# MD-0022). And after cultured in the medium for 5-7 days, these cells will express high levels of CHAT and MAP2 (Figure 2).
Figure 1. After cultured in Motor Neuron Maintenance Medium on the Matrigel-coated plates for 2 days, more than 85% of the iPSC-derived motor neurons express HB9 (Figure A and A’), and more than 90% of the cells express ISL1 (Figure B and B’).
Figure 2. After cultured in Motor Neuron Maintenance Medium on the Matrigel-coated plates for 5-7 days, more than 85% of the iPSC-derived motor neurons express ChAT (Figure A) and MAP2 (Figure B).
Figure 3. Co-culture of iXCells™ hiPSC-derived motor neurons with mouse astrocytes for 7 days. Motor neurons are stained with Tuj1, and mouse astrocytes are stained with GFAP.
|Human iPSC-derived motor neurons (Normal)|
|1.0 million cells/vial; 2.0 million cells/vial; 4.0 million cells/vial (frozen)|
|Human Motor Neuron Maintenance Medium (Cat # MD-0022)|
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