Figure 1. Representative high content screening images for cells treated with (A) vehicle control (B) 50 µM chloroquine (a lysosomotropic agent) and (C) 150 µM piroxicam (a non-lysosomotropic agent) over a 4 hr period. Drugs which are lysosomotropic such as chloroquine competitively inhibit uptake of the lysosomal dye into the lysosomes.
Figure 2. Graphical representation of lysosomal trapping data for chloroquine (a lysosomotropic agent) and piroxicam (a non-lysosomotropic agent). Chloroquine causes a concentration dependent decrease in lysosomal staining compared to vehicle control treated cells. No effect was observed for piroxicam. No cytotoxic effects were observed for either compound at the concentration range tested. Data represents mean of triplicate incubations ± standard deviation.