Mouse Adipose-Derived Stem Cells-brown fat (MADSC-bf)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10MU-005
$359.00

Product Description

Adipose-derived stem cells (ADSC) are multipotent mesenchymal stem cells (MSC) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc. in vitro. ADSC have been applied in studies including stem cell differentiation, regenerative medicine [1], cell therapy, tissue engineering and creation of iPS cell lines.

iXCells Biotechnologies provides high quality Mouse Adipose-Derived Stem Cells, also knowns as preadipocytes, which are isolated from C57BL/6 mouse inguinal white fat tissue or interscapular brown fat tissue. These cells are cryopreserved at P1, with >0.5 million cells in each vial and can further expand for 3-4 population doublings in Adipose-derived Stem Cell Growth Medium (Cat # MD-0003) under the condition suggested by iXCells Biotechnologies. In vitro, mADSC can be differentiated into adipocytes and osteoblasts (Figure 1 and 2) using Adipocyte Differentiation Medium (Cat # MD-0005) and Osteogenic Differentiation Medium (Cat # MD-0006), separately. These mADSC can be further expanded for no more than 3 passages using Adipose-derived Stem Cell Growth Medium (Cat # MD-0003). These cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

 

Adipocyte differentiation

 Figure 1. (A) Mouse ADSCs (phase contrast). (B) Adipocyte induction (Day 10 post adipogenic induction, phase contrast). (C) Adipocyte induction (Day 10 post adipogenic induction, Oil Red O staining).

 

Osteogenic induction

Figure 2.  Mouse ADSCs can be differentiated to into osteoblasts using the osteogenic induction protocol (Alizarin Red S staining, Day 21 post osteogenic induction).

 

Product Details

  Tissue   C57BL/6 mouse interscapular brown fat tissue
  Package Size   0.5 million cells/vial  
  Passage Number   P1
  Shipped   Cryopreserved 
  Storage   Liquid nitrogen
  Growth Properties   Adherent
  Media

  Adipose-derived Stem Cell Growth Medium (Cat # MD-0003)

  Adipocyte Differentiation Medium (Cat # MD-0005)

  Osteogenic Differentiation Medium (Cat # MD-0006)
 
 
References
 
[1]  Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.
Biological
Cell Type Adipose-Derived Stem Cells
Species Mouse