Mouse Adipose-Derived Stem Cells-white fat (MADSC-wf)

Product Description

Adipose-derived stem cells (ADSC) are multipotent mesenchymal stem cells (MSC) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc. in vitro. ADSC have been applied in studies including stem cell differentiation, regenerative medicine ^[1], cell therapy, tissue engineering and creation of iPS cell lines.

iXCells Biotechnologies provides high quality Mouse Adipose-Derived Stem Cells, also knowns as preadipocytes, which are isolated from C57BL/6 mouse inguinal white fat tissue or interscapular brown fat tissue. These cells are cryopreserved at P1, with >0.5 million cells in each vial and can further expand for 3-4 population doublings in Adipose-derived Stem Cell Growth Medium (Cat # MD-0003) under the condition suggested by iXCells Biotechnologies. In vitro, mADSC can be differentiated into adipocytes and osteoblasts (Figure 1 and 2) using Adipocyte Differentiation Medium (Cat # MD-0005) and Osteogenic Differentiation Medium (Cat # MD-0006), separately. These mADSC can be further expanded for no more than 3 passages using Adipose-derived Stem Cell Growth Medium (Cat # MD-0003). These cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

 Figure 1. (A) Mouse ADSCs (phase contrast). (B) Adipocyte induction (Day 10 post adipogenic induction, phase contrast). (C) Adipocyte induction (Day 10 post adipogenic induction, Oil Red O staining).

Figure 2.  Mouse ADSCs can be differentiated to into osteoblasts using the osteogenic induction protocol (Alizarin Red S staining, Day 21 post osteogenic induction).

Product Details

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 Adipocyte Differentiation Protocol

 

 Osteogenic Differentiation Protocol