Mouse Dermal Fibroblasts - neonatal (MDFB-n)
Fibroblasts are the most common cells in connective tissue, and their main function is to continuously secreting extracelluar matrix proteins such as collagens, glycoproteins and glycosaminoglycans to maintain the structural integrity of the connective tissue. Dermal fibroblasts play critical role during wound healing by producing extracellular matrix and wound healing mediators [1,2]. Therefore, dermal fibroblasts are well suited for wound healing studies. They can be used for wound healing studies and dermatological research to investigate various skin diseases. Additionally, fibroblasts are important for tissue regeneration, cancer research and tissue engineering studies.
iXCells Biotechnologies provides high quality primary Mouse Dermal Fibroblasts-neonatal (MDFB-n), which are isolated from the dermis of neonatal mouse skin and cryopreserved at P1, with >1 million cells in each vial. MDFB-n are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand for 1 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies.
Neonatal mouse skin
1.0 million cells/vial (Enough to seed on one T75 and ready to subculture in 3 days for assay)
Note: please do not allow cells to be overgrown (passage the cells at 70~85% confluency).
|Media||Fibroblast Growth Medium (Cat# MD-0011)|
 Akita S, Akino K, Imaizumi T, Hirano A. Wound Repair Regen.(2008),16(5):635-641. Basic fibroblast growth factor accelerates and improves second-degree burn wound healing.
 Nolte SV1, Xu W, Rennekampff HO, Rodemann HP. Cells Tissues Organs. (2008);187(3):165-76. Diversity of fibroblasts--a review on implications for skin tissue engineering.
Koo, J., Jang, H., Lee, Y., Moon, Y. J., Bae, E. J., Yun, S., & Park, B. (2019). Myeloid cell-specific sirtuin 6 DEFICIENCY delays wound healing in mice by Modulating inflammation and Macrophage phenotypes. Experimental & Molecular Medicine, 51(4), 1-10. doi:10.1038/s12276-019-0248-9 -- Learn More
Wang, Z., Mascarenhas, N., Eckmann, L., Miyamoto, Y., Sun, X., Kawakami, T., & Di Nardo, A. (2017). Skin microbiome promotes mast cell maturation by triggering stem cell factor production in keratinocytes. Journal of Allergy and Clinical Immunology, 139(4). doi:10.1016/j.jaci.2016.09.019 -- Learn More