Mouse Hepatic Stellate Cells (MHSC)

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10MU-021

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Product Description

Hepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCsHepatic stellate cells (HSCs) are liver-specific mesenchymal cells, and account for 5~8% of the cells in the liver. HSCsplay vital roles in the homeostasis of liver extracellular matrix, repair, regeneration and fibrosis, and control retinolmetabolism, storage and release. The stellate cell is the major cell type involved in liver fibrosis in response to liver injury[1]. In healthy liver, HSCs are in a quiescent state, and contains numerous vitamin A lipid droplets, constituting the largestreservoir of vitamin A in the body. When the liver is damaged, HSCs can change into an activated state, which ischaracterized by proliferation, contractility and chemotaxis. The amount of vitamin A decreases progressively in injuredliver. The activated HSCs also secrete collagen scar tissue, which can lead to cirrhosis. In chronic liver disease,prolonged and repeated activation of stellate cells causes liver fibrosis [2]. Primary culture of HSC is a valuable tool tostudy liver fibrosis.

 

iXCells Biotechnologies provides high quality frozen or freshly plated mouse HSCs (MHSC) isolated from mouse liver.iXCells Biotechnologies provides high quality frozen or freshly plated mouse HSCs (MHSC) isolated from mouse liver.The cells are frozen immediately after isolation, with >0.5 million cells per vial. There will be 30-50% dead cells afterthawing, which is normal for MHSC. We don’t recommend passaging the MHSCs.

 

Mouse Hepatic Stellate Cells

Figure 1. (A) Phase contrast image of day 3 culture of mouse hepatic stellate cells (MHSC).  (B). Vitamin A droplets in MHSC were visualized by blue autofluorescence (lower panel) when excited with UV light.

 

Product Details

  Tissue   Mouse liver 
  Package Size   0.5 million cells/vial (enough to seed on 3 wells of a 12-well plate).
  Passage Number   P0
  Shipped   Fresh or frozen
  Storage   Liquid nitrogen
  Growth Properties   Adherent
  Media   Stellate Cell Growth Medium (Cat# MD-0014)

 

 
References
[1] Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.

[2] Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.
 

 

[1]  Chunyue Yin, et al, J Clin Invest. 2013;123(5):1902–1910. Hepatic stellate cells in liver development, regeneration, and cancer.
[2]  Rockey D. C. . Semin Liver Dis 21(3):337-49. (2001) Hepatic blood flow regulation by stellate cells in normal and injured liver.

Biological
Cell Type Stellate Cells
Species Mouse