Rat Cortical Neurons (RCN)

Product Description

Cortical neurons make up a large portion of the brain and consist of both glutamateric and GABA-ergic neurons ^[1].  They play an important role in higher level thought processes and are necessary to organize input from subcortical structures.   Like all neurons, these cells transport Na+ that work to propagate action potentials from one cell to the next, which is the basis of a working neural pathway.  These cells can be used to study a variety of cortical related abnormalities including Huntington’s Disease, Alzheimer’s Disease, psychiatric disorders, and learning disorders ^{[2, 3]}.

iXCells Biotechnologies provides high quality Rat Cortical Neurons (RCN), which are isolated from the brains of E16 embryonic rats.  These cells are cryopreserved at P0, with ≥ 2 million cells in each vial.  They are negative for mycoplasma, bacteria, yeast, and fungi. RCNs can be plated using Rat Cortical Neuron Recovery Medium (Cat# MD-0107A) and maintained in Rat Cortical Neuron Maintenance Medium (Cat # MD-0107B) under the condition suggested by iXCells Biotechnologies.

Figure 1. Rat Cortical Neuron (Cat# 10RA-032) were recovered and cultured for 5 days. (A) Phase contrast image. (B) Immunofluorescence staining were performed using antibodies against the neuronal markers MAP2 (green) and NeuN (red). 

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Reference

[1] Johnson M, Walsh C. (2017) " Cerebral Cortical Neuron Diversity and Development at Single-Cell Resolution " Curr Opin Neurobiol. 42: 9-16.

[2] Lim L, Mi D, Llorca A, Marin O. (2018) " Development and functional diversification of cortical interneurons." Neuron. 100(2): 294-313.

[3] Molnár Z, Clowry G J, Šestan N, Alzu’bi A, Bakken T, Hevner R F, et al. (2019) " New insights into the development of the human cerebral cortex." J. Anat. 235: 432-451.

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