Rat Lymphatic Fibroblasts (RLF)

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Description Product Code Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10RA-019

Product Description

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in various organs are intrinsically different [2]. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids [3].

iXCells Biotechnologies provides high quality Rat Lymphatic Fibroblasts (RLF), which are isolated from rat lymph node and cryopreserved at P0, with >0.5 million cells in each vial. RLF are characterized by their spindle-shaped morphology. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Rat lymph node

  Package Size

  0.5 million cells/vial  

  Passage Number

  P0

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Fibroblast Growth Medium (Cat# MD-0011)

References

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. 
[3] Domracheva, E. V., Rogova, E. M., Vorob'ev, A. L. (1984) Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. 
[3] Domracheva, E. V., Rogova, E. M., Vorob'ev, A. L. (1984) Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.

Biological
Cell Type Fibroblasts
Species Rat